Mouse Anti-GABARAP Recombinant Antibody (VS3-CJ188) (CAT#: VS3-CJ188)

• Immunogen
• Purified His-tagged GABARAP protein (Fragment)

• Host Species
• Mouse

• Type
• Mouse IgG1

• Specificity
• Human and Mouse GABARAP

• Species Reactivity
• Human, Mouse

• Applications
• WB

• Conjugate
• Unconjugated

• Purification
• Protein G affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• PBS

• Preservative
• 0.09% sodium azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in Western Blot (1:1000).

• Alternative Names
• MM46; ATG8A; GABARAP-a

• Gene ID
• 11337

• UniProt ID
• O95166

• Sequence Similarities
• Belongs to the ATG8 family.

• Cellular Localization
• Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Golgi apparatus, Membrane, Microtubule

• Post Translation Modifications
• The precursor molecule is cleaved by ATG4 (ATG4A, ATG4B, ATG4C or ATG4D) to expose the glycine at the C-terminus and form the cytosolic form, GABARAP-I (PubMed:15169837, PubMed:20818167, PubMed:30661429).
  The processed form is then activated by APG7L/ATG7, transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) phospholipid to form the membrane-bound form, GABARAP-II (PubMed:15169837).
  During non-canonical autophagy, the processed form is conjugated to phosphatidylserine (PS) phospholipid (PubMed:33909989).
  ATG4 proteins also mediate the delipidation of PE-conjugated forms (PubMed:33909989).
  In addition, ATG4B and ATG4D mediate delipidation of ATG8 proteins conjugated to PS during non-canonical autophagy (PubMed:33909989).
  ATG4B constitutes the major protein for proteolytic activation (PubMed:30661429).
  ATG4D is the main enzyme for delipidation activity (By similarity).
  (Microbial infection) The Legionella effector RavZ is a deconjugating enzyme that hydrolyzes the amide bond between the C-terminal glycine residue and an adjacent aromatic residue in ATG8 proteins conjugated to phosphatidylethanolamine (PE), producing an ATG8 protein that is resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine (PubMed:31722778).
  RavZ is also able to mediate delipidation of ATG8 proteins conjugated to phosphatidylserine (PS) (PubMed:33909989).

• Protein Refseq
• NP_009209.1

• Function
• Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton (PubMed:9892355).
  Involved in autophagy: while LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:15169837, PubMed:20562859, PubMed:22948227).
  Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006538).
  Also required for the local activation of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex, regulating ubiquitination and degradation of TIAM1, a guanyl-nucleotide exchange factor (GEF) that activates RAC1 and downstream signal transduction (PubMed:25684205).
  Thereby, regulates different biological processes including the organization of the cytoskeleton, cell migration and proliferation (PubMed:25684205).
  Involved in apoptosis (PubMed:15977068).

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody

Secondary Antibody

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS