Mouse Anti-GABRG2 Recombinant Antibody (clone 10F10-C1-B8) (CAT#: FAMAB-1353CQ)

Figure 1 Detection of g2 subunit in a Western blot assay using 10F10-C1-B8 antibody.

(A) 10, 25, 50, and 100 μg rat or mouse hippocampal proteins were Western blotted using 2 μg/mL of 10F10-C1-B8 antibody. Arrowhead indicates γ2 signal. Position of molecular weight marker 30, 40, and 50 kDa is also shown for reference (lane M). (B) Expression of γ2 subunit in 10-100 μg rat or mouse hippocampal proteins in a Western blotting assay using 2 μg/mL rabbit polyclonal antibody. Arrowhead indicates γ2 signal. Lane M shows position of marker proteins of 30, 40, and 50 kDa sizes. (C) Expression of rat γ2L subunit in HEK293 cells (lanes T) transfected with a cDNA expressing rat γ2L subunit. 75 μg total proteins were Western blotted using 10F10-C1-B8 antibody (2 μg/mL, panel Ms) or rabbit polyclonal antibody (2 μg/mL, panel Rb). Proteins from untransfected cells were used as control (lanes NT). Position of molecular weight proteins 40 and 50 kDa is also shown (lane M). (D) γ2 subunit was immunoprecipitated from 500 μg hippocampal proteins using 100 μg of 10F10-C1-B8 antibody (lane Ms), a rabbit polyclonal antibody against γ2 subunit (lane Rb), or normal mouse IgG (lane MsIgG). Arrowhead indicates γ2 signal. In lane M, molecular weight marker, 40 and 50 kDa is marked.

Figure 2 Immunohistochemical detection of γ2 subunit using 10F10-C1-B8 antibody.

Immunohistochemical detection of γ2 subunit in a control neuron not expressing siRNA and grown in vitro for 8 days.