Rabbit Anti-GATA3 Recombinant Antibody (clone 2A9) (CAT#: ZG-0011U)
The purified Rabbit Anti-GATA3 Recombinant Antibody (clone 2A9) displayed high specificity against the GATA3 protein of human.
Specific Inquiry
WB
Figure 1 Rabbit Anti-GATA3 Antibody (ZG-0011U) in WB
Positive WB detected in: RAW264.7 whole cell lysate. All lanes: GATA3 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 48, 49 kDa
Observed band size: 48 kDa
IHC
Figure 2 Rabbit Anti-GATA3 Antibody (ZG-0011U) in IHC
IHC image of ZG-0011U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human GATA3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human GATA3
- Species Reactivity
- Human
- Clone
- 2A9
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Hypoparathyroidism, Sensorineural Deafness, And Renal Disease and End Stage Renal Disease
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunohistochemistry. Specific conditions should be optimized by the end user. WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- Trans-acting T-cell-specific transcription factor GATA-3;GATA-binding factor 3; GATA3
- Gene ID
- 2625
- UniProt ID
- P23771
Related Resources
Inflammatory Bowel Diseases
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "GATA3"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2040MZ | Recombinant Mouse Anti-Human GATA3 Antibody (clone 1A12-1D9) | ELISA, WB | Mouse antibody |
| ZG-0596F | Mouse Anti-GATA3 Recombinant Antibody (clone L50-823) | IHC-P | Mouse IgG1, κ |
| VS3-CJ803 | Mouse Anti-GATA3 Recombinant Antibody (VS3-CJ803) | WB, IF, FC | Mouse IgG2b, κ |
| VS3-CJ804 | Rabbit Anti-GATA3 (phospho S308) Recombinant Antibody (VS3-CJ804) | WB, IHC, IP | Rabbit IgG |
| VS3-XY762 | Mouse Anti-GATA3 Recombinant Antibody (clone 1A10D1) | ELISA, WB | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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