Recombinant Human Anti-GM3 Antibody (L612) (CAT#: HPAB-0190CQ)
This product is a recombinant human antibody that recognizes GM3. L612, a human IgM monoclonal antibody produced by an EBV-transformed human B-cell line, binds to ganglioside GM3 and kills GM3-positive human melanoma cells in the presence of complement.
Specific Inquiry
WB
Figure 1 Western blot analysis of purified protein.
To detect reactivity with L612 (e), the blotted membrane was incubated with 20 μg/ml L612 in TPBS and binding of L612 detected with peroxidase conjugated anti-human IgM. The amount of antibody loaded in each lane was 2 jig for protein staining and 0.2 μgfor Western blot analysis
Hastings, A., Morrison, S. L., Kanda, S., Saxton, R. E., & Irie, R. F. (1992). Production and characterization of a murine/human chimeric anti-idiotype antibody that mimics ganglioside. Cancer research, 52(7), 1681-1686.
Inhib
Figure 2 Direct binding and competitive inhibition assays of murine and chimeric anti-Id binding to L612.
ELISA plates were coated with L612 at a concentration of 80 μg/min 0.1 M carbonate buffer, pH 9.6.
Hastings, A., Morrison, S. L., Kanda, S., Saxton, R. E., & Irie, R. F. (1992). Production and characterization of a murine/human chimeric anti-idiotype antibody that mimics ganglioside. Cancer research, 52(7), 1681-1686.
ELISA
Figure 3 Cell-ELISA inhibition assay of chimeric and murine anti-idiotypic antibodies.
Plates were coated with membranes prepared from melanoma cells expressing GM3. L612 was added to each well at a concentration of 8 μg/ml; 4C10 and TVE1 diluted from 16 to 0.125 μg/ml were added as inhibitors and the binding of L612 determined using peroxidase conjugated anti-human IgM.
Hastings, A., Morrison, S. L., Kanda, S., Saxton, R. E., & Irie, R. F. (1992). Production and characterization of a murine/human chimeric anti-idiotype antibody that mimics ganglioside. Cancer research, 52(7), 1681-1686.
Specifications
- Host Species
- Human
- Derivation
- EBV-transformed human B-cell line
- Type
- Human IgM
- Specificity
- GM3
- Clone
- L612
- Applications
- ELISA, WB, Inhib
- Related Disease
- Cutaneous malignant melanoma
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Inhibition and Enzyme-linked Immunosorbent Assay. Please see the Published Data for further information.
Target
- Alternative Names
- Ganglioside GM3; GM3; monosialodihexosylganglioside
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone L612"
See other products for "GM3"
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-407-F(E) | Recombinant Anti-GM3 Antibody Fab Fragment | IF, FuncS | Fab |
| MHH-407-F(E) | Recombinant Human Anti-GM3 Antibody Fab Fragment | WB, IF, FuncS | Fab |
| PFBC-051 | Mouse Anti-GM3 Recombinant Antibody (clone 14F7); Fab Fragment | ELISA | Mouse Fab |
| HPAB-0190CQ-F(E) | Recombinant Human Anti-GM3 Antibody Fab Fragment (L612) | ELISA | Human Fab |
| FAMAB-0055WJ-F(E) | Human Anti-GM3 Recombinant Antibody (clone L612); Fab Fragment | WB | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-407-S(P) | Recombinant Anti-GM3 Antibody scFv Fragment | WB, Neut, FuncS | scFv |
| MHH-407-S(P) | Recombinant Human Anti-GM3 Antibody scFv Fragment | WB, FC, FuncS | scFv |
| PSBC-051 | Mouse Anti-GM3 Recombinant Antibody (clone 14F7); scFv Fragment | ELISA | Mouse scFv |
| HPAB-0190CQ-S(P) | Recombinant Human Anti-GM3 Antibody scFv Fragment (L612) | ELISA | Human scFv |
| FAMAB-0055WJ-S(P) | Human Anti-GM3 Recombinant Antibody (clone L612); scFv Fragment | WB | Human scFv |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-407 | Recombinant Human Anti-GM3 Antibody | IP, IF, Biosensors, FuncS | IgG |
| PABC-051 | Mouse Anti-GM3 Recombinant Antibody (clone 14F7) | ELISA | Mouse IgG |
| FAMAB-0055WJ | Human Anti-GM3 Recombinant Antibody (clone L612) | WB, FC, Cyt | Human IgM |
| VS-0224-XY52 | Mouse Anti-GM3 Recombinant Antibody (clone GMR6) | ELISA, FC, IHC | Mouse IgM, kappa |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-321CL | Human Anti-GM3 Recombinant Antibody (TAB-321CL) | ELISA | Human IgM |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0308MC | Recombinant Mouse Anti-GM3 Antibody | TLC-immunostaining, Flow cytometric analysis, Enzyme-Linked Immunosorbent Assay, IHC |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for HPAB-0190CQ. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.