This human anti-GNB5 antibody (clone H9) is a product that recognizes Human guanine nucleotide-binding protein beta 5 (GBB5).
Figure 1 ELISA of scFv-displayed virions against the GBB5 peptide.
ScFv antibodies, raised against the GBB5 peptide, are displayed on phage particles and used to probe peptides, which are captured on microtiter plate well surface.
Figure 2 Five scFvs in a soluble ELISA retain their specificity.
After transferring the scFv coding region from the phagemid into an expression vector, these five scFvs were expressed in soluble form and purified. To demonstrate the specificity of each antibody, the ELISA was performed against all biomarker peptides available.
Figure 3 Photonic crystal binding curve.
The anti-GBB5-scFv H9 was titrated across wells of a streptavidincoated, photonic crystal, 96-well, biosensor plate, with the GBB5 peptide immobilized on the well surface. The half maximal effective concentration (EC50) of ~ 300 nM was used to
estimate affinity.
Figure 4 Western blot of retinal lysates.
The anti-GBB5-scFv H9 was used to probe retinal lysates from chicken, mouse, and rabbit. The left panel is probed with the scFv antibody and the secondary anti-FLAG-HRP antibody. The right panel serves as the negative control (i.e., probed with the secondary antibody only).
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1743MZ | Recombinant Mouse Anti-Human GNB5 Antibody | WB | Mouse antibody |
VS-0323-CJ24 | Human Anti-GNB5 Recombinant Antibody (clone A1) | ELISA, WB | Human IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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