Mouse Anti-GPC4 Recombinant Antibody (clone 09) (CAT#: VS-0224-XY55)
The antibody is a mouse monoclonal antibody specific for human GPC4 (Glypican 4). It was purified by protein A or G affinity chromatography. This product has been detected by Enzyme-linked immunosorbent assay and Flow cytometry.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line RPTEC TERT1
(Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30834/1789_A11_1_cr5961fc340c048_selected.jpg
(Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30834/1789_A11_1_cr5961fc340c048_selected.jpg
Figure 2 Cerebral cortex
(Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30836/65282_B_8_5.jpg
(Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30836/65282_B_8_5.jpg
Figure 3 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30836/65282_A_7_3.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30836/65282_A_7_3.jpg
Figure 4 Liver
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30836/65282_A_7_4.jpg
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30836/65282_A_7_4.jpg
Figure 5 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30836/65282_A_9_5.jpg
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30836/65282_A_9_5.jpg
Figure 6 Testis
(Elongated or late spermatids Staining: Low Intensity: Moderate Quantity: <25% Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30836/65282_A_6_6.jpg
(Elongated or late spermatids Staining: Low Intensity: Moderate Quantity: <25% Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30836/65282_A_6_6.jpg
Figure 7 RNA cell line category: Cell line enhanced (AF22, HAP1, NTERA-2, RPTEC TERT1, SuSa)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000076716-GPC4
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000076716-GPC4
Specifications
- Immunogen
- CHO-derived recombinant human Glypican 4 (AA 1-529)
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human GPC4
- Species Reactivity
- Human
- Clone
- clone 09
- Applications
- ELISA, Flow Cytometry
- Related Disease
- Keipert Syndrome; Simpson-Golabi-Behmel Syndrome, Type 1
Product Property
- Purification
- Protein A or G purified
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested in ELISA and Flow cytometry (0.25 µg/106 cells).
Target
- Alternative Names
- Glypican 4; Glypican Proteoglycan 4; K-Glypican; DJ900E8.1 (Glypican 4); Glypican-4
- Gene ID
- 2239
- UniProt ID
- O75487
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "GPC4"
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1487 | Hi-Affi™ Rabbit Anti-GPC4 Recombinant Antibody (clone DS1487AB) | IHC-P | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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