Mouse Anti-GST Recombinant Antibody (clone G196) (CAT#: PABC-388)

Figure 1 Western blot analysis of FLAG-HA-Emerald GFP (FHG) tagged with C-terminal G196-tag in HeLa cells using mAb G196.

HeLa cells were transfected with FHG/pCDNA3 or EHG-G196/pcDNA3. After incubation for two days. the cells were lysed and subiected to Western blotting with anti-FLAG (M2) or G196 mAbs.

Tatsumi, K., Sakashita, G., Nariai, Y., Okazaki, K., Kato, H., Obayashi, E., ... & Urano, T. (2017). G196 epitope tag system: a novel monoclonal antibody, G196, recognizes the small, soluble peptide DLVPR with high affinity. Scientific reports, 7, 43480.

Figure 2 Western blot analysis of Atfl-G196-GFP in fission yeast using mAb G196.

A DNA fragment containing the transcription factor atfl gene fused with the G196 epitope-GFP gene was replaced with the genomic atfl gene. Protein extracts were separated by SDS-PAGE and detected with mAb G196 and anti-GFP polyclonal antibody. GFP-Iwsl expressing cells were used as a negative control.

Tatsumi, K., Sakashita, G., Nariai, Y., Okazaki, K., Kato, H., Obayashi, E., ... & Urano, T. (2017). G196 epitope tag system: a novel monoclonal antibody, G196, recognizes the small, soluble peptide DLVPR with high affinity. Scientific reports, 7, 43480.

Figure 3 Immunoprecipitation (IP) analysis of FLAG-HA-Emerald GFP (FHG) tagged with C-terminal G196-tag in HeLa cells using mAb G196.

HeLa cells were transfected with FHG/pCDNA3 or EHG-G196/pcDNA3. After incubation for two days. the cells were lysed and these lysates were immunoprecipitated with anti-FLAG (M2) (lanes 3, 4) or G196 (lanes 5, 6) mAbs and subiected to Western blotting with anti-FLAG polyclonal antibody.

Tatsumi, K., Sakashita, G., Nariai, Y., Okazaki, K., Kato, H., Obayashi, E., ... & Urano, T. (2017). G196 epitope tag system: a novel monoclonal antibody, G196, recognizes the small, soluble peptide DLVPR with high affinity. Scientific reports, 7, 43480.

Figure 4 Immunofluorescence of GFP- and G196-tagged nuclear protein in fssion yeast cells using mAb G196.

Atf1-G196-GFP expressing cells were fxed and incubated with mAb G196 and anti-GFP polyclonal Ab, then stained with Alexa-594 anti-mouse and Alexa-488 anti-rabbit secondary antibodies.

Tatsumi, K., Sakashita, G., Nariai, Y., Okazaki, K., Kato, H., Obayashi, E., ... & Urano, T. (2017). G196 epitope tag system: a novel monoclonal antibody, G196, recognizes the small, soluble peptide DLVPR with high affinity. Scientific reports, 7, 43480.

Figure 5 Immunofluorescence of GFP- and G196-tagged nuclear protein in HeLa cells using mAb G196.

HeLa cells were transfected with FHG-G196-NAC1/pcDNA3. Afer incubation for two days, the cells were fxed and incubated with mAb G196 and anti-GFP polyclonal Ab, then stained with Alexa-594 anti-mouse and Alexa-488 anti-rabbit secondary antibodies.

Tatsumi, K., Sakashita, G., Nariai, Y., Okazaki, K., Kato, H., Obayashi, E., ... & Urano, T. (2017). G196 epitope tag system: a novel monoclonal antibody, G196, recognizes the small, soluble peptide DLVPR with high affinity. Scientific reports, 7, 43480.