Popular Searches: Recombinant Antibody MHC Tetramer T cell Receptor Scaffold Protein
  • Home
  • GTF2B
  • Mouse Anti-GTF2B Recombinant Antibody (VS3-CJ27)

Mouse Anti-GTF2B Recombinant Antibody (VS3-CJ27) (CAT#: VS3-CJ27)

This product is a mouse antibody that recognizes GTF2B.


See more Engineered Antibody products for GTF2B
Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Figure 1 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61626/1153_H2_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human rectum
Figure 2 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61626/ihc_selected.jpg
Figure 3 Cerebral cortex
Figure 3 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61626/140313_B_9_5.jpg
Figure 4 Colon
Figure 4 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61626/140313_A_9_3.jpg
Figure 5 Kidney
Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61626/140313_A_8_5.jpg
Figure 6 Testis
Figure 6 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Peritubular cells Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61626/140313_A_4_6.jpg
Figure 7 Lymph node
Figure 7 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61626/140313_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000137947-GTF2B

Specifications

  • Immunogen
  • Recombinant full length of human GTF2B
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human GTF2B
  • Species Reactivity
  • Human
  • Applications
  • WB, ELISA
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Protein G affinity purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Format
  • Liquid
  • Concentration
  • 1 mg/mL (lot specific)
  • Buffer
  • 50% Glycerol, 1% BSA, PBS, pH7.4.
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:500-1:1000).

Target

  • Alternative Names
  • TF2B; TFIIB
  • Sequence Similarities
  • Belongs to the TFIIB family.
  • Cellular Localization
  • Chromosome, Nucleus
  • Post Translation Modifications
  • Acetylated (PubMed:24441171).
    Autoacetylated; autoacetylation at Lys-238 stimulates transcription activation (PubMed:12931194).
  • Function
  • General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:1876184, PubMed:1946368, PubMed:1517211, PubMed:3818643, PubMed:3029109, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:7601352, PubMed:9420329, PubMed:12931194, PubMed:27193682).
    Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8504927, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312).
    Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352).
    Associates with chromatin to core promoter-specific regions (PubMed:12931194, PubMed:24441171).
    Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element (PubMed:9420329, PubMed:16230532, PubMed:7675079, PubMed:10619841).
    Modulates transcription start site selection (PubMed:10318856).
    Exhibits also autoacetyltransferase activity that contributes to the activated transcription (PubMed:12931194).

Recommended Products

Isotype Control

Secondary Antibody

Recommended Dilution Buffer

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "GTF2B"

Recombinant Antibody

CAT Product Name Application Type
MOB-2012CT Recombinant Mouse anti-Human GTF2B Monoclonal antibody (JJC2) ChIP, IP, WB
VS3-QX489 Mouse Anti-GTF2B Recombinant Antibody (clone 2F6-A3) WB Mouse IgG2b
VS4-WK206 Mouse Anti-GTF2B Recombinant Antibody (VS4-WK206) ELISA, WB, IHC, ICC Mouse IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1539 Rabbit Anti-GTF2B Recombinant Antibody (clone DS1539AB) WB, IHC, IP, FC Rabbit IgG

Customer Reviews and Q&As

Submit a review or a question
There are currently no Customer reviews or questions for VS3-CJ27. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

© 2024 Creative Biolabs.
  • 0
  • 0
Cart
    Inquiry

    Go to compare