Popular Searches: Recombinant Antibody MHC Tetramer T cell Receptor Scaffold Protein
  • Home
  • HINT1
  • Mouse Anti-HINT1 Recombinant Antibody (VS3-CJ995)

Mouse Anti-HINT1 Recombinant Antibody (VS3-CJ995) (CAT#: VS3-CJ995)

This product is a mouse antibody that recognizes human, mouse, and rat HINT1.


See more Engineered Antibody products for HINT1
Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44577/if_selected.jpg
Normal Tissue
Figure 2 Colon
Figure 2 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44577/98115_A_7_3.jpg
Figure 3 Pancreas
Figure 3 Pancreas
(Exocrine glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Pancreatic endocrine cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44577/98115_A_2_3.jpg
Figure 4 Kidney
Figure 4 Kidney
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44577/98115_A_8_5.jpg
Figure 5 Testis
Figure 5 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44577/98115_A_4_6.jpg
Figure 6 Lymph node
Figure 6 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44577/98115_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169567-HINT1

Specifications

  • Immunogen
  • Recombinant protein of human HINT1
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1, κ
  • Specificity
  • Human, Mouse, Rat HINT1
  • Species Reactivity
  • Human, Mouse, Rat
  • Applications
  • WB, ICC, IHC, FC
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Affinity purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Format
  • Liquid
  • Buffer
  • PBS
  • Preservative
  • 0.09% Sodium Azide
  • Storage
  • Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Western Blot (1:4000), Immunocytochemistry (1:25), Immunohistochemistry (1:25), Flow Cytometry (1:25).

Target

  • Alternative Names
  • HINT; NMAN; PKCI-1; PRKCNH1
  • Sequence Similarities
  • Belongs to the HINT family.
  • Cellular Localization
  • Cytoplasm, Nucleus
  • Function
  • Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2 (PubMed:15703176, PubMed:16835243, PubMed:17337452, PubMed:17217311, PubMed:23614568, PubMed:28691797, PubMed:29787766, PubMed:31990367, PubMed:22329685).
    Hydrolyzes adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate) (PubMed:15703176, PubMed:16835243).
    Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His-AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester (PubMed:15703176, PubMed:22329685, PubMed:17337452).
    Hydrolyzes 3-indolepropionic acyl-adenylate, tryptamine adenosine phosphoramidate monoester and other fluorogenic purine nucleoside tryptamine phosphoramidates in vitro (PubMed:17337452, PubMed:23614568, PubMed:29787766, PubMed:17217311, PubMed:28691797, PubMed:31990367).
    Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O-phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide (PubMed:30772266).
    In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1 (PubMed:16014379, PubMed:22647378).
    Modulates p53/TP53 levels and p53/TP53-mediated apoptosis (PubMed:16835243).
    Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin-protein ligase complex (PubMed:19112177).
    Also exhibits SUMO-specific isopeptidase activity, deconjugating SUMO1 from RGS17 (PubMed:31088288).
    Deconjugates SUMO1 from RANGAP1 (By similarity).

Recommended Products

Secondary Antibody

Recommended Dilution Buffer

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "HINT1"

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1597 Rabbit Anti-HINT1 Recombinant Antibody (clone DS1597AB) WB, IHC-P, ICC, FC Rabbit IgG

Recombinant Antibody

CAT Product Name Application Type
VS3-XY806 Mouse Anti-HINT1 Recombinant Antibody (clone 4G12) WB, IP, IF Mouse IgG1

Customer Reviews and Q&As

Submit a review or a question
There are currently no Customer reviews or questions for VS3-CJ995. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

© 2024 Creative Biolabs.
  • 0
  • 0
Cart
    Inquiry

    Go to compare