Mouse Anti-HIV-1 gp160 Recombinant Antibody (clone D19) (CAT#: MRO-3851CQ)

Figure 1 ELISAs were performed by using recombinant gp120 in the solid phase in the presence or absence of sCD4.

The specificity of the reactions was verified by preadsorption of MAb D19 with a full-length V3-derived peptide.

Lusso, P., Earl, P. L., Sironi, F., Santoro, F., Ripamonti, C., Scarlatti, G., ... & Burastero, S. E. (2005). Cryptic nature of a conserved, CD4-inducible V3 loop neutralization epitope in the native envelope glycoprotein oligomer of CCR5-restricted, but not CXCR4-using, primary human immunodeficiency virus type 1 strains. Journal of virology, 79(11), 6957-6968.

Figure 2 Fluorocytometric analysis of MAb D19 binding to the native Env expressed on the surface of cells persistently infected with different HIV-1 strains.

The cells, PM1 or SupT1, were persistently
infected with HIV-1 isolates of different coreceptor preference: IIIB
(X4).

Lusso, P., Earl, P. L., Sironi, F., Santoro, F., Ripamonti, C., Scarlatti, G., ... & Burastero, S. E. (2005). Cryptic nature of a conserved, CD4-inducible V3 loop neutralization epitope in the native envelope glycoprotein oligomer of CCR5-restricted, but not CXCR4-using, primary human immunodeficiency virus type 1 strains. Journal of virology, 79(11), 6957-6968.

Figure 3 Neutralization of different HIV-1 isolates by MAb D19 in the presence or absence of sCD4.

Phytohemagglutinin-activated human
PBMC, previously treated or not with MAb D19 at the indicated concentrations,
were acutely infected with isolates BaL.

Lusso, P., Earl, P. L., Sironi, F., Santoro, F., Ripamonti, C., Scarlatti, G., ... & Burastero, S. E. (2005). Cryptic nature of a conserved, CD4-inducible V3 loop neutralization epitope in the native envelope glycoprotein oligomer of CCR5-restricted, but not CXCR4-using, primary human immunodeficiency virus type 1 strains. Journal of virology, 79(11), 6957-6968.