Human Anti-HLA-DR10 Recombinant Antibody (clone ChLym-1) (CAT#: HPAB-0617-WJ)
This product is a humanized antibody targeting HLA-DR10. This antibody can be used for the manufacture of a medicament for treatment of leukemia.
Specific Inquiry
Inhib
Figure 1 Growth inhibitory effect on Raji cells.
At 48 hours, chLym-1 (-□-) caused a significant growth inhibitory effect over the range of 0.01–0.13 μM. Wells incubated with 0.13 μM of chLym-1 contained 90% fewer cells than untreated cells. LeLPLDB (-●-), (Leac-PLD)2PLDB (-○-), and ItPLDB (-■-) caused no growth-in-hibitory effect over the same concentration range.
West, J., Perkins, J., Hok, S., Balhorn, R., Lightstone, F. C., Cosman, M., ... & DeNardo, G. L. (2006). Direct antilymphoma activity of novel, first-generation" antibody mimics" that bind HLA-DR10-positive non-Hodgkin's lymphoma cells. Cancer biotherapy & radiopharmaceuticals, 21(6), 645-654.
Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG
- Specificity
- Human HLA-DR10
- Species Reactivity
- Human
- Clone
- ChLym-1
- Applications
- Inhib
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported/tested for use in Inhibition Assay.
Inhib: Twenty (20)-, 2-, and 0.2-μM LeLPLDB, (Leac-PLD)2LPB, and ItPLDB solutions were preparedin RPMI-1640, supplemented with 10% heat-in-activated FBS. Aliquots of the 20-μM solutionswere removed and incubated with 1:100 SAHRP for 1 hour at ambient temperature before serial dilution. Raji and B35M cells were suspended at a concentration of 500,000 cells/mL in the samemedium. Negative controls were prepared, con-taining concentrations of biotin, SAHRP, and di-methylsulfoxide (DMSO) equal to that of the SHAL dilutions. One hundred (100) μL of both the SHAL solutions and the cell solutions were added in duplicate to 96-well plates. The plates were incubated at 37°C in a 5% CO₂ atmosphereand removed for counting at 24, 48, and 72 hours.At each time point, 100 μL was removed from each well and 10 μL of trypan blue was added and mixed, using gentle pipeting. Ten (10) μL of the resulting solution was added to each side of a hemocytometer, and the cells were counted using a light microscope under 100× magnification. Viable cells appeared colorless, whereasdead cells appeared blue because of uptake of try-pan blue dye. Wells containing chLym-1 at concentrations of 0.17, 0.33, 3.3, 1.7, 33, 67, and 133 nM were used as positive controls.
Target
- Alternative Names
- HLA-DR10; DR10
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone ChLym-1"
See other products for "HLA-DR10"
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-362CL | Mouse Anti-HLA-DR10 Recombinant Antibody (TAB-362CL) | WB, IHC | Mouse IgG2a |
| TAB-096CT | Anti-Human HLA-DR10 Recombinant Antibody (Lym-1) | FC, IF, Inhib, IHC | |
| TAB-096CT-S(P) | Anti-Human HLA-DR10 Recombinant Antibody scFv Fragment (Lym-1) | ELISA, WB | |
| TAB-096CT-F(E) | Anti-Human HLA-DR10 Recombinant Antibody Fab Fragment (Lym-1) | ELISA, WB |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0617-WJ-F(E) | Human Anti-HLA-DR10 Recombinant Antibody (clone ChLym-1); Fab Fragment | Block, FC | Humanized Fab |
| HPAB-1183-FY-S(P) | Human Anti-HLA-DR10 Recombinant Antibody (clone FY-003); Fab Fragment | Inhib | Humanized Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0617-WJ-S(P) | Human Anti-HLA-DR10 Recombinant Antibody (clone ChLym-1); scFv Fragment | Block, FC | Humanized scFv |
| HPAB-1183-FY-F(E) | Human Anti-HLA-DR10 Recombinant Antibody (clone FY-003); scFv Fragment | Inhib | Humanized scFv |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1183-FY | Human Anti-HLA-DR10 Recombinant Antibody (clone FY-003) | Inhib | Humanized IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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