Mouse Anti-HMOX1 Recombinant Antibody (clone 3F9) (CAT#: VS3-WK1594)
This antibody is a mouse recombinant antibody against human HMOX1, which is an essential enzyme in heme catabolism The clone 3F9 can be used in WB analysis at a dilution of 1/1000-1/2000 and IHC analysis at a dilution of 1/50-1/200.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/635/51_B8_2_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/635/51_B8_2_selected.jpg
Figure 2 Colon
(Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17444/39144_A_9_3.jpg
(Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17444/39144_A_9_3.jpg
Figure 3 Kidney
(Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17444/39144_A_7_5.jpg
(Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17444/39144_A_7_5.jpg
Figure 4 Lymph node
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17444/39144_A_7_8.jpg
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17444/39144_A_7_8.jpg
Figure 5 High expression in spleen
(Cells in red pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17444/39144_B_8_4.jpg
(Cells in red pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17444/39144_B_8_4.jpg
Figure 6 RNA cell line category: Cell line enhanced (BEWO, GAMG, U-138 MG, U-87 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100292-HMOX1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100292-HMOX1
Specifications
- Immunogen
- Synthesized peptide derived from human HO-1
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human HMOX1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 3F9
- Applications
- IHC-P, WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 0.5% BSA, 50% Glycerol
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- IHC-P: 1/50-1/200
WB: 1/1000-1/2000
Target
- Alternative Names
- Heme Oxygenase 1; Heme Oxygenase (Decycling) 1; HO-1; Heat Shock Protein, 32-KD; EC 1.14.14.18; EC 1.14.99.3
- Gene ID
- 3162
- UniProt ID
- P09601
- Long Name
- Heme Oxygenase 1
- Sequence Similarities
- Belongs to the heme oxygenase family.
- Cellular Localization
- Endoplasmic reticulum, Membrane
- Post Translation Modifications
- A soluble form arises by proteolytic removal of the membrane anchor.
- Protein Refseq
- NP_002124.1
- Function
- Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "HMOX1"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0877CT | Recombinant Mouse anti-Rat HMOX1 Monoclonal antibody (IP-2-3) | ELISA, WB | |
| MRO-0728-CN | Rabbit Anti-HMOX1 Recombinant Antibody (clone CBACN-265) | WB, IHC, IP, FC | Rabbit IgG |
| MRO-1925-CN | Rabbit Anti-HMOX1 Polyclonal Antibody (MRO-1925-CN) | WB, IF, IHC | Rabbit IgG |
| VS3-CJ541 | Rabbit Anti-HMOX1 Recombinant Antibody (VS3-CJ541) | WB, IHC, IP, FC | Rabbit IgG |
| VS3-FY687 | Recombinant Rabbit Anti-HMOX1 Antibody (clone R02-6C1) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-912CQ | Mouse Anti-HMOX1 Recombinant Antibody (clone GTS-1) | ELISA, IHC-Fr, IHC-P, Inhib, WB | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1639 | Rabbit Anti-HMOX1 Recombinant Antibody (clone DS1639AB) | WB, IHC, ICC, IP | Rabbit IgG |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0841 | PE-A*02:01/Human HMOX1 (QLFEELQEL) MHC Tetramer | FCM, IHC | |
| MHC-CN0842 | APC-A*02:01/Human HMOX1 (QLFEELQEL) MHC Tetramer | FCM, IHC | |
| MHC-CN0843 | FITC-A*02:01/Human HMOX1 (QLFEELQEL) MHC Tetramer | FCM, IHC | |
| MHC-CN0844 | A*02:01/Human HMOX1 (QLFEELQEL) MHC Tetramer | FCM, IHC | |
| MHC-CN0845 | PE-A*03:01/Human HMOX1 (QVLKKIAQK) MHC Tetramer | FCM, IHC |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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