Rabbit Anti-HPGD Recombinant Antibody (clone R04-5I2) (CAT#: VS3-XY3385)
The antibody is a recombinant rabbit antibody binding with HPGD (15-hydroxyprostaglandin dehydrogenase), which catalyzes the NAD-dependent dehydrogenation of a broad array of hydroxylated polyunsaturated fatty acids. This product recognizes and binds to human HPGD. It has been detected using Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5679/10_A5_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5679/10_A5_1_selected.jpg
Figure 2 Testis
(Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5679/20208_A_4_6.jpg
(Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5679/20208_A_4_6.jpg
Figure 3 Colon
(Endocrine cells Staining: Medium Intensity: Moderate Enterocytes Staining: Medium Intensity: Moderate Quantity:>75% Enterocytes -)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5679/20208_A_7_3.jpg
(Endocrine cells Staining: Medium Intensity: Moderate Enterocytes Staining: Medium Intensity: Moderate Quantity:>75% Enterocytes -)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5679/20208_A_7_3.jpg
Figure 4 Liver
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5679/20208_A_9_4.jpg
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5679/20208_A_9_4.jpg
Figure 5 Kidney
(Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5679/20208_A_9_5.jpg
(Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5679/20208_A_9_5.jpg
Figure 6 Urinary bladder
(Urothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5679/20208_A_5_5.jpg
(Urothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5679/20208_A_5_5.jpg
Figure 7 RNA cell line category: Cell line enhanced (A549, ASC diff, HEL, OE19, RT4, SuSa)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000164120-HPGD
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000164120-HPGD
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human HPGD
- Species Reactivity
- Human
- Clone
- R04-5I2
- Applications
- WB, IHC-P, ICC, IF, FC
- Related Disease
- Hypertrophic Osteoarthropathy, Primary, Autosomal Recessive, 1
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- PGDH; PGDH1; PHOAR1; 15-PGDH; SDR36C1
- Gene ID
- 3248
- UniProt ID
- P15428
- Long Name
- 15-hydroxyprostaglandin dehydrogenase
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (P15428)
Modification sites at neXtProt (NX_P15428)
- Protein Refseq
- NP_000851.2
- Function
- Catalyzes the NAD-dependent dehydrogenation (oxidation) of a broad array of hydroxylated polyunsaturated fatty acids (mainly eicosanoids and docosanoids, including prostaglandins, lipoxins and resolvins), yielding their corresponding keto (oxo) metabolites (PubMed:8086429, 10837478, 16828555, 16757471, 21916491, 25586183).
Decreases the levels of the pro-proliferative prostaglandins such as prostaglandin E2 (whose activity is increased in cancer because of an increase in the expression of cyclooxygenase 2) and generates oxo-fatty acid products that can profoundly influence cell function by abrogating pro-inflammatory cytokine expression (PubMed:25586183, 15574495).
Converts resolvins E1, D1 and D2 to their oxo products, which represents a mode of resolvin inactivation.
Resolvin E1 plays important roles during the resolution phase of acute inflammation, while resolvins D1 and D2 have a unique role in obesity-induced adipose inflammation.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "HPGD"
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1669 | Rabbit Anti-HPGD Recombinant Antibody (clone DS1669AB) | WB | Rabbit IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-279YJL | Rabbit Anti-HPGD Recombinant Antibody (clone CBH264) | WB, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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