Mouse Anti-HSPA5 Recombinant Antibody (ZG-0333F) (CAT#: ZG-0333F)

This product is a mouse antibody that recognizes the epitope C-terminus of the HSPA5.


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WB

Figure 1 Mouse Anti-HSPA5 Recombinant Antibody (ZG-0333F) in WB.

Figure 1 Mouse Anti-HSPA5 Recombinant Antibody (ZG-0333F) in WB.

Western blot detection of BiP/GRP78 (C-terminal) in Hela, C6, Lncap and MDA-MB-468 cell lysates using BiP/GRP78 (C-terminal) mouse mAb (1:1000 dilution). Predicted band size: 72KDa. Observed band size: 78KDa.

IF

Figure 2 Mouse Anti-HSPA5 Recombinant Antibody (ZG-0333F) in IF.

Figure 2 Mouse Anti-HSPA5 Recombinant Antibody (ZG-0333F) in IF.

Immunocytochemical staining was performed on HeLa cells fixed with 4% paraformaldehyde, and anti-BiP/GRP78 (C-terminal) mouse mAb (diluted 1:50) was used.

IHC

Figure 3 Mouse Anti-HSPA5 Recombinant Antibody (ZG-0333F) in IHC.

Figure 3 Mouse Anti-HSPA5 Recombinant Antibody (ZG-0333F) in IHC.

Immunohistochemical analysis of paraffin-embedded colorectal cancer was performed using BiP/GRP78 (C-terminal) mouse mAb (1/100 dilution). Antigen retrieval is performed by pressure cooking in citrate buffer (pH 6.0).


Specifications

  • Immunogen
  • Recombinant human BiP/GRP78 (C-terminus) protein fragments expressed in E. coli.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human HSPA5
  • Species Reactivity
  • Human, Rat
  • Applications
  • Western Blot, Immunocytochemistry
  • Conjugate
  • NONE

Product Property

  • Format
  • Liquid
  • Concentration
  • 1 mg/mL
  • Storage
  • Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1:1000
    Immunocytochemistry: 1:50

Target

  • Alternative Names
  • Heat Shock Protein Family A (Hsp70) Member 5; Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa); Endoplasmic Reticulum Lumenal Ca(2+)-Binding Protein Grp78; Immunoglobulin Heavy Chain-Binding Protein; Glucose-Regulated Protein, 78kDa; GRP78; BIP; Heat Shock 70kD Protein 5 (Glucose-Regulated Protein, 78kD); 78 kDa glucose regulated protein; 78 kDa glucose-regulated reticulum lumenal Ca(2)-binding protein grp78; Endoplasmic reticulum lumenal Ca2 binding protein grp78; FLJ26106; Glucose Regulated Protein 78kDa; GRP 78; GRP-78; GRP78; GRP78_HUMAN; Heat shock 70 kDa protein 5; Heat Shock 70kDa Protein 5; Hsce70; HSPA 5; HSPA5; Immunoglobulin Heavy Chain Binding Protein; Immunoglobulin heavy chain-binding protein; mBiP; MIF2; Sez7.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "HSPA5"

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1695 Rabbit Anti-HSPA5 Recombinant Antibody (clone DS1695AB) WB, IHC-P Rabbit IgG

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-0368-YJ-S(P) Mouse Anti-HSPA5 Recombinant Antibody (clone MAb159); scFv Fragment WB, FC, IHC, ELISA, Inhib, FuncS Mouse scFv
HPAB-0369-YJ-S(P) Human Anti-HSPA5 Recombinant Antibody (clone huMAb159); scFv Fragment WB, FC, IHC, ELISA, Inhib, FuncS Humanized scFv

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-0368-YJ-F(E) Mouse Anti-HSPA5 Recombinant Antibody (clone MAb159); Fab Fragment WB, FC, IHC, ELISA, Inhib, FuncS Mouse Fab
HPAB-0369-YJ-F(E) Human Anti-HSPA5 Recombinant Antibody (clone huMAb159); Fab Fragment WB, FC, IHC, ELISA, Inhib, FuncS Humanized Fab

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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