Mouse Anti-IAV HA Recombinant Antibody (clone 8H10) (CAT#: PABJ-0096)

This product is a Mouse antibody that recognizes IAV HA. Passive transfer of a murine IgG1 version of 8H10 conferred limited protection against 5xLD50 intranasal challenge of H3N2 X31, but the murine IgG2c version of 8H10 conferred complete protection against the same challenge regimen. An Fc-dependent mechanism was responsible for protection.


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SDS-PAGE

Figure 1 Mouse Anti-IAV HA Recombinant Antibody (clone 8H10) (PABJ-0096) in SDS-PAGE

Figure 1 Mouse Anti-IAV HA Recombinant Antibody (clone 8H10) (PABJ-0096) in SDS-PAGE

SDS-PAGE analysis of PABJ-0096 in non-reduced (Lane 2) and reduced (Lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 130-180 kDa.

SEC-HPLC

Figure 2 Mouse Anti-IAV HA Recombinant Antibody (clone 8H10) (PABJ-0096) in SEC-HPLC

Figure 2 Mouse Anti-IAV HA Recombinant Antibody (clone 8H10) (PABJ-0096) in SEC-HPLC

HPLC analysis of PABJ-0096 shows the purity is>95%.

WB

Figure 3 Human Anti-IAV H3N2 HA Recombinant Antibody (PABJ-0096) in WB

Figure 3 Human Anti-IAV H3N2 HA Recombinant Antibody (PABJ-0096) in WB

Western blot analysis of PABJ-0096 was performed with Recombinant Influenza A H3N2 (A/Texas/50/2012) Hemagglutinin Protein (HA1 Subunit) (His Tag).
Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)
The secondary antibody: HRP Goat Anti-Human IgG (H+L)


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG2c
  • Specificity
  • IAV HA
  • Species Reactivity
  • Influenza A virus
  • Clone
  • 8H10
  • Applications
  • ELISA, FuncS
  • Related Disease
  • Influenza

Product Property

  • Purification
  • Protein A/G Purified
  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Buffer
  • PBS
  • Storage
  • Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Functional Assay.

Target

  • Alternative Names
  • Influenza A virus Hemagglutinin; Influenza A virus; Hemagglutinin; HA; IAV

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone 8H10"

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Human Antibody

CAT Product Name Application Type
TAB-H33 Anti-IAV HA Recombinant Antibody (Firivumab) FC, IP, ELISA, Neut, FuncS, IF, ICC IgG1 - kappa
TAB-027ML Anti-IAV HA Recombinant Antibody (Gedivumab) ELISA, IHC, FC, IP, IF, FuncS IgG1, κ
TAB-438CQ Anti-IAV HA Recombinant Antibody (Navivumab) ELISA, IHC, FC, IP, IF, BL IgG1, κ

Epitope-Specific Antibody

CAT Product Name Application Type
EPAF-0652CQ Recombinant Mouse Anti-IAV HA Antibody (pSAN13.4.1) ELISA IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-027ML Afuco™ Anti-IAV HA ADCC Recombinant Antibody (Gedivumab), ADCC Enhanced ELISA, IHC, FC, IP, IF, FuncS ADCC enhanced antibody
AFC-TAB-438CQ Afuco™ Anti-IAV HA ADCC Recombinant Antibody (Navivumab), ADCC Enhanced ELISA, IHC, FC, IP, IF, BL ADCC enhanced antibody
AFC-TAB-H33 Afuco™ Anti-IAV HA ADCC Recombinant Antibody (Firivumab), ADCC Enhanced FC, IP, ELISA, Neut, FuncS, IF ADCC enhanced antibody

MHC Tetramer for Virology

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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