Rabbit Anti-IGHG1 Recombinant Antibody (clone R29-3R-6) (CAT#: VS3-FY2150)
Clone R29-3R-6 is a rabbit IgG recombinant monoclonal antibody that demonstrates specificity in binding to human IGHG1. Western blot demonstrated that R29-3R-6 recognized a target protein with a molecular weight of 44 kDa.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R29-3R-6 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000).
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_9_3.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_9_3.jpg
Figure 2 Liver
(Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_9_4.jpg
(Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_9_4.jpg
Figure 3 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_8_5.jpg
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_8_5.jpg
Figure 4 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_5_6.jpg
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_5_6.jpg
Figure 5 RNA cell line category: Group enriched (Karpas-707, U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000211896-IGHG1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000211896-IGHG1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human IGHG1
- Species Reactivity
- Human
- Clone
- R29-3R-6
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 44kDa, while the observed molecular weight stands at 52kDa.
- Related Disease
- Leukemia, Chronic Lymphocytic and Heavy Chain Deposition Disease
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Immunoglobulin Heavy Constant Gamma 1 (G1m Marker)
- Gene ID
- 299354
- UniProt ID
- P01857
- Cellular Localization
- Cell membrane, Immunoglobulin, Membrane, Secreted
- Post Translation Modifications
- Glycosylation on Asn-180 is required for interaction with Fc receptors and ability to activate the complement pathway. (P01857-IGHG1_HUMAN)
(Microbial infection) Deglycosylation on Asn-180 by S.pyogenes EndoS or Endos2 endoglucosidases prevents interaction between immunoglobulin-gamma (IgG) and Fc receptors, impairing ability to activate the complement pathway. (P01857-IGHG1_HUMAN)
Glycosylation at Asn180 (NX_P01857 [NX_P01857-1])
Modification sites at PhosphoSitePlus (P01857)
Glycosylation from GlyConnect IGHG1_HUMAN (233, 2976, 2977, 2980, 2981, 2982, 2983, 2984, 2985, 3002, 671)
Glycosylation from GlyGen (P01857) 2 sites, 189 N-linked glycans (2 sites), 1 O-linked glycan (1 site)
- Function
- Mediates IgG effector functions on monocytes triggering ADCC of virus-infected cells.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1117z | Mouse Anti-IGHG1 Recombinant Antibody (clone 5C2) | FC, IF, IHC, WB | Mouse IgG1, κ |
| MOB-034LC | Mouse Anti-IGHG1 Recombinant Antibody (clone YC121) | ELISA, WB, IHC | Mouse IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1394 | Rabbit Anti-IGHG1 Recombinant Antibody (clone DS1394AB) | ICC, IHC, FC, ELISA | Rabbit IgG |
| MOR-1771 | Rabbit Anti-IGHG1 Recombinant Antibody (clone DS1771AB), PE | FC | Rabbit IgG |
Epitope-Specific Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0577CQ | Mouse Anti-IGHG1 Recombinant Antibody (clone B3-18) | ELISA | Mouse IgM |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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