Rabbit Anti-IKZF1 Recombinant Antibody (clone R08-5E4) (CAT#: VS3-FY1747)

• Immunogen
• Synthetic peptide of human icaros

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Hamster IKZF1

• Species Reactivity
• Human, Hamster

• Clone
• R08-5E4

• Applications
• Western Blot, Immunohistochemistry-Paraffin

• MW
• The calculated weight of the target protein is approximately 58 kDa, while the observed molecular weight stands at 50-70 kDa.

• Related Disease
• Immunodeficiency, Common Variable, 13 and Diamond-Blackfan Anemia-Like

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry: 1/50-1/100

• Alternative Names
• IKAROS Family Zinc Finger 1

• Gene ID
• 10320

• UniProt ID
• Q13422

• Sequence Similarities
• Belongs to the Ikaros C2H2-type zinc-finger protein family.

• Cellular Localization
• Cytoplasm, Nucleus

• Post Translation Modifications
• Phosphorylation controls cell-cycle progression from late G1 stage to S stage. Hyperphosphorylated during G2/M phase. Dephosphorylated state during late G1 phase. Phosphorylation on Thr-140 is required for DNA and pericentromeric location during mitosis. CK2 is the main kinase, in vitro. GSK3 and CDK may also contribute to phosphorylation of the C-terminal serine and threonine residues. Phosphorylation on these C-terminal residues reduces the DNA-binding ability. Phosphorylation/dephosphorylation events on Ser-13 and Ser-295 regulate TDT expression during thymocyte differentiation. Dephosphorylation by protein phosphatase 1 regulates stability and pericentromeric heterochromatin location. Phosphorylated in both lymphoid and non-lymphoid tissues (By similarity).
  Phosphorylation at Ser-361 and Ser-364 downstream of SYK induces nuclear translocation.

• Protein Refseq
• XP_011513360.1; XP_011513361.1; XP_011513362.1; XP_011513363.1; XP_011513364.1

• Function
• Transcription regulator of hematopoietic cell differentiation.
  Binds gamma-satellite DNA.
  Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity).

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody