Human Anti-IL12 Recombinant Antibody (clone Y61) (CAT#: HPAB-0844-FY)
This product is a recombinant antibody that recognizes IL12. The monoclonal antibody Y61 reacts with the epitope of IL12.
Specific Inquiry
FuncS
Figure 1 Anti-CII antibody isotype distribution in anti-IL-12- and control rIgG-treated IFN- R KO mice.
Animals were immunized with CII in CFA (day 0) and were bled on day 28.
Matthys, P., Vermeire, K., Mitera, T., Heremans, H., Huang, S., & Billiau, A. (1998). Anti‐IL‐12 antibody prevents the development and progression of collagen‐induced arthritis in IFN‐γ receptor‐deficient mice. European journal of immunology, 28(7), 2143-2151.
Inhib
Figure 2 Effect of anti-IL-12 antibody treatment on the cytokine secretion profile of wild-type mice after an antiCD3 challenge.
The data of one out of two experiments, with similar results, are shown.
Matthys, P., Vermeire, K., Mitera, T., Heremans, H., Huang, S., & Billiau, A. (1998). Anti‐IL‐12 antibody prevents the development and progression of collagen‐induced arthritis in IFN‐γ receptor‐deficient mice. European journal of immunology, 28(7), 2143-2151.
IHC
Figure 3 Effect of anti-IL-12 antibody on the microscopic findings and terminal deoxynucleotidyltransferase dUTP nick end labeling (TUNEL) results in murine lungs after BLM instillation.
These pathological changes and positive signals for TUNEL t 14 days were abrogated by the administration of 200 mg/animal of anti-IL-12 antibody.
Maeyama, T., Kuwano, K., Kawasaki, M., Kunitake, R., Hagimoto, N., & Hara, N. (2001). Attenuation of bleomycin-induced pneumopathy in mice by monoclonal antibody to interleukin-12. American Journal of Physiology-Lung Cellular and Molecular Physiology, 280(6), L1128-L1137.
WB
Figure 4 Western blot analysis of anti-IL12 in lung tissue.
Data shown with standard error bars are from one of three experiments with similar results.
Maeyama, T., Kuwano, K., Kawasaki, M., Kunitake, R., Hagimoto, N., & Hara, N. (2001). Attenuation of bleomycin-induced pneumopathy in mice by monoclonal antibody to interleukin-12. American Journal of Physiology-Lung Cellular and Molecular Physiology, 280(6), L1128-L1137.
ELISA
Figure 5 Results of semiquantification of mRNA expression in lung tissue.
Optical density (OD) values for each individual fragment were normalized to b-actin expression from the same tissue fragment.
Maeyama, T., Kuwano, K., Kawasaki, M., Kunitake, R., Hagimoto, N., & Hara, N. (2001). Attenuation of bleomycin-induced pneumopathy in mice by monoclonal antibody to interleukin-12. American Journal of Physiology-Lung Cellular and Molecular Physiology, 280(6), L1128-L1137.
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human IL12
- Species Reactivity
- Human
- Clone
- Y61
- Applications
- Inhib, WB, IHC, ELISA, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The IL12 antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Western Blot, Inhibition, Immunohistochemistry, Functional Assay.
Target
- Alternative Names
- Interleukin 12; IL12
Related Resources
Asthma
Inflammatory Bowel Diseases
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone Y61"
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-602 | Human Anti-IL12 Recombinant Antibody (clone CNTO1275) | Neut, ELISA | Humanized IgG |
| MOB-016CQ | Mouse Anti-IL12 Recombinant Antibody (clone YC123) | ELISA, FC, FuncS, ELISAPOT | Mouse IgG1 |
| MOB-Z041-YC | Human Anti-IL12 Recombinant Antibody (clone DEC.YC041) | WB, ELISA, IF, FC | Human IgG1, κ |
| HPAB-1551WJ | Human Anti-IL12 Recombinant Antibody (clone 70-1) | ELISA, WB | Human IgG1 |
| HPAB-1552WJ | Human Anti-IL12 Recombinant Antibody (clone 101-11) | ELISA, WB | Human IgG1 |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-597 | Human Anti-IL12 Recombinant Antibody (clone CNTO1275); Fab Fragment | Neut, ELISA | Humanized Fab |
| HPAB-0844-FY-S(P) | Human Anti-IL12 Recombinant Antibody (clone Y61); Fab Fragment | Neut | Human Fab |
| HPAB-1551WJ-F(E) | Human Anti-IL12 Recombinant Antibody; Fab Fragment (clone 70-1) | ELISA, WB | Human Fab |
| HPAB-1552WJ-F(E) | Human Anti-IL12 Recombinant Antibody; Fab Fragment (clone 101-11) | ELISA, WB | Human Fab |
| HPAB-1554WJ-F(E) | Human Anti-IL12 Recombinant Antibody; Fab Fragment (clone 78-34) | ELISA, WB | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-597 | Human Anti-IL12 Recombinant Antibody (clone CNTO1275); scFv Fragment | Neut, ELISA | Humanized scFv |
| HPAB-0844-FY-F(E) | Human Anti-IL12 Recombinant Antibody (clone Y61); scFv Fragment | Neut | Human scFv |
| HPAB-1551WJ-S(P) | Human Anti-IL12 Recombinant Antibody; scFv Fragment (clone 70-1) | ELISA, WB | Human scFv |
| HPAB-1552WJ-S(P) | Human Anti-IL12 Recombinant Antibody; scFv Fragment (clone 101-11) | ELISA, WB | Human scFv |
| HPAB-1554WJ-S(P) | Human Anti-IL12 Recombinant Antibody; scFv Fragment (clone 78-34) | ELISA, WB | Human scFv |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1151CQ | Rat Anti-IL12 Recombinant Antibody (clone 20C2) | Block, ELISA, WB | Rat IgG1 |
| NEUT-1153CQ | Rat Anti-IL12 Recombinant Antibody (clone C8.6) | Block, ELISA, WB | Rat IgG1 |
| NEUT-1162CQ | Mouse Anti-IL12 Recombinant Antibody (clone B-T21) | ELISA, Block | Mouse IgG1 |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1152CQ | Mouse Anti-IL12 Recombinant Antibody (clone 31H23) | Neut, WB | Mouse IgG1 |
| NEUT-1154CQ | Mouse Anti-IL12 Recombinant Antibody (clone CBL092) | Neut, WB, IHC | Mouse IgG1 |
| NEUT-1155CQ | Mouse Anti-IL12 Recombinant Antibody (clone CBL244) | ELISA, Neut | Mouse IgG1 |
| NEUT-1156CQ | Rat Anti-IL12 Recombinant Antibody (clone 9A5) | Block, ELISA, ICC, IF, IHC, IP, WB | Rat IgG2b |
| NEUT-1157CQ | Rat Anti-Il12 Recombinant Antibody (clone mAb0853) | WB, IP, Neut, FC | Rat IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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