Rabbit Anti-ISG15 Recombinant Antibody (clone R06-6K4) (CAT#: VS3-XY3398)
This antibody is a recombinant rabbit antibody targeting the ISG15 (ISG15 ubiquitin like modifier), which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein. It specifically reacts with human ISG15. It has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin and Immunofluorescence is 1/500-1/1000, 1/50-1/100 and 1/50-1/200, respectively.
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 IHC staining of human liver
(Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/ihc_selected.jpg
(Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/ihc_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_B_7_5.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_B_7_5.jpg
Figure 3 Colon
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_A_8_3.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_A_8_3.jpg
Figure 4 Liver
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_A_8_4.jpg
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_A_8_4.jpg
Figure 5 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_A_7_5.jpg
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_A_7_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_A_4_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_A_4_6.jpg
Figure 7 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_A_9_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_A_9_8.jpg
Figure 8 RNA cell line category: Cell line enhanced (BJ hTERT+ SV40 Large T+, BJ hTERT+ SV40 Large T+ RasG12V, HeLa, SK-MEL-30)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000187608-ISG15
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000187608-ISG15
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ISG15
- Species Reactivity
- Human
- Clone
- R06-6K4
- Applications
- WB, IHC-P, ICC, IF
- Related Disease
- Immunodeficiency 38 With Basal Ganglia Calcification
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
Target
- Alternative Names
- G1P2; IP17; UCRP; IFI15; IMD38; hUCRP
- Gene ID
- 9636
- UniProt ID
- P05161
- Long Name
- ISG15 ubiquitin like modifier
- Cellular Localization
- Cytoplasm, Secreted
- Post Translation Modifications
- Ubiquitination at Lys35 and Lys77 (NX_P05161 [NX_P05161-1])
Modification sites at PhosphoSitePlus (P05161)
Glycosylation from GlyGen (P05161) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_005092.1
- Function
- Ubiquitin-like protein which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein.
ISGylation involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes which catalyze the conjugation of ISG15 to a lysine residue in the target protein.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1007MZ | Recombinant Mouse Anti-Human ISG15 Antibody (clone 4F6) | ELISA | Mouse antibody |
| MOB-125CQ | Mouse Anti-Mouse ISG15 Antibody | ELISA, WB, IP, IF | Mouse IgG2a, κ |
| VS3-CJ400 | Mouse Anti-ISG15 Recombinant Antibody (VS3-CJ400) | WB | |
| VS4-WK253 | Rabbit Anti-ISG15 Recombinant Antibody (clone 16D8) | ELISA, IHC, FC | Rabbit IgG |
| VS3-FY767 | Recombinant Rabbit Anti-ISG15 Antibody (clone R08-1H5) | WB | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1866 | Hi-Affi™ Recombinant Rabbit Anti-ISG15 Monoclonal Antibody (DS1866AB) | ICC/IF, WB | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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