Rabbit Anti-KDM1A Recombinant Antibody (clone 1G7) (CAT#: ZG-0784J)
This product is a rabbit antibody that recognizes KDM1A.
Specific Inquiry
WB
Figure 1 Rabbit Anti-KDM1 Antibody (ZG-0784J) in WB
Western Blot
Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate, PC-3 whole cell lysate, K562 whole cell lysate
All lanes: KDM1A antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 93, 96 kDa
Observed band size: 110 kDa
IHC
Figure 2 Rabbit Anti-KDM1 Antibody (ZG-0784J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC
Figure 3 Rabbit Anti-KDM1 Antibody (ZG-0784J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human KDM1 / LSD1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human KDM1A
- Species Reactivity
- Human
- Clone
- 1G7
- Applications
- ELISA, WB, IHC
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- AOF2; CPRF; KDM1; LSD1; BHC110; lysine-specific histone demethylase 1A; BRAF35-HDAC complex protein BHC110; FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit; [histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A; amine oxidase (flavin containing) domain 2; flavin-containing amine oxidase domain-containing protein 2; lysine (K)-specific demethylase 1A; lysine-specific histone demethylase 1
- Gene ID
- 23028
- UniProt ID
- O60341
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "KDM1A"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2167MZ | Recombinant Mouse Anti-Human KDM1A Antibody | ELISA, ELISA, ICC/IF, IP, WB | Mouse antibody |
| MRO-2018-CN | Rabbit Anti-KDM1A Polyclonal Antibody (MRO-2018-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-QX634 | Mouse Anti-KDM1A Recombinant Antibody (clone 1E5-H2) | WB, ICC, IP | Mouse IgG1 |
| VS3-CJ10 | Mouse Anti-KDM1A Recombinant Antibody (VS3-CJ10) | WB, ELISA | Mouse IgG |
| VS3-XY960 | Mouse Anti-KDM1A Recombinant Antibody (clone 3G1) | WB, IP, IF | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1924 | Hi-Affi™ Recombinant Rabbit Anti-KDM1A Monoclonal Antibody (DS1924AB) | WB, IHC, ICC, IP | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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