Rabbit Anti-KDM1A Recombinant Antibody (clone R08-3J5) (CAT#: VS3-FY1755)

• Immunogen
• Synthetic peptide of human KDM1/LSD1

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Hamster, Rat KDM1A

• Species Reactivity
• Human, Hamster, Rat

• Clone
• R08-3J5

• Applications
• Western Blot, Immunofluorescence, Immunoprecipitation

• MW
• The calculated weight of the target protein is approximately 93 kDa, while the observed molecular weight stands at 110 kDa.

• Related Disease
• Cleft Palate, Psychomotor Retardation, And Distinctive Facial Features and Neuroblastoma

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunofluorescence: 1/50-1/200
  Immunoprecipitation: 1/20

• Alternative Names
• Lysine Demethylase 1A; Flavin-Containing Amine Oxidase Domain-Containing Protein 2; Amine Oxidase (Flavin Containing) Domain 2; BRAF35-HDAC Complex Protein BHC110; AOF2; KDM1; LSD1; FAD-Binding Protein BRAF35-HDAC Complex, 110 KDa Subunit; Lysine-Specific Histone Demethylase 1A

• Gene ID
• 23028

• UniProt ID
• O60341

• Sequence Similarities
• Belongs to the flavin monoamine oxidase family.

• Cellular Localization
• Nucleus

• Post Translation Modifications
• Polyubiquitinated by JADE2; which leads to its proteasomal degradation (PubMed:25018020).
  Deubiquitinated by USP38; preventing it from degradation by the 26S proteasome (PubMed:30497519).

• Protein Refseq
• XP_005245843.1; XP_006710536.1; XP_016856205.1; XP_047305633.1; XP_054191226.1

• Function
• Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me.
  May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity.
  Also acts as a coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in AR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody