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  • Rabbit Anti-KDM2A Recombinant Antibody (clone R05-4D6)

Rabbit Anti-KDM2A Recombinant Antibody (clone R05-4D6) (CAT#: VS3-FY1715)

Clone R05-4D6 is a rabbit recombinant monoclonal antibody that is designed to target human KDM2A. We recommend antibody dilution: western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). Western blot demonstrated that R05-4D6 recognized a target protein with a molecular weight of 133 kDa.


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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44251/if_selected.jpg
Figure 2 IHC staining of human stomach
Figure 2 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12272/ihc_selected.jpg
Figure 3 IF staining of human cell line A-431
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44251/813_A7_1_red_green.jpg
Figure 4 IF staining of human cell line U-251 MG
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44251/754_A7_3_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Figure 5 Cerebral cortex
(Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glial cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12272/33160_B_8_5.jpg
Figure 6 Stomach
Figure 6 Stomach
(Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12272/33160_A_4_1.jpg
Figure 7 Colon
Figure 7 Colon
(Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12272/33160_A_7_3.jpg
Figure 8 Kidney
Figure 8 Kidney
(Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12272/33160_A_8_5.jpg
Figure 9 Testis
Figure 9 Testis
(Elongated or late spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Leydig cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Peritubular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Preleptotene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12272/33160_A_6_6.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000173120-KDM2A

Specifications

  • Immunogen
  • Synthetic peptide of human FBXL11
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human KDM2A
  • Species Reactivity
  • Human
  • Clone
  • R05-4D6
  • Applications
  • Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
  • MW
  • The calculated weight of the target protein is approximately 133 kDa, while the observed molecular weight stands at 133 kDa.
  • Related Disease
  • Duodenum Adenoma and Ossifying Fibromyxoid Tumor

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry: 1/50-1/100
    Immunofluorescence: 1/50-1/200
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • Lysine Demethylase 2A; F-Box And Leucine-Rich Repeat Protein 11; JmjC Domain-Containing Histone Demethylation Protein 1A; Jumonji C Domain-Containing Histone Demethylase 1A; [Histone-H3]-Lysine-36 Demethylase 1A; Lysine (K)-Specific Demethylase 2A; CXXC-Type Zinc Finger Protein 8; F-Box/LRR-Repeat Protein 11; EC 1.14.11.27; FBXL11; JHDM1A
  • Sequence Similarities
  • Belongs to the JHDM1 histone demethylase family.
  • Cellular Localization
  • Chromosome, Nucleus
  • Post Translation Modifications
  • Mono-ADP-ribosylated at Arg-1020 in response to DNA damage, leading to displacement from chromatin, resulting in increased dimethylation of histone H3 at 'Lys-36'.
  • Function
  • Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere.

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "KDM2A"

Recombinant Antibody

CAT Product Name Application Type
DrMAB-365 Recombinant Human Anti-Human KDM2A Antibody, clone D435 IF, ELISA, IP-MS IgG

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