Rabbit Anti-LGALS3 Recombinant Antibody (clone 4E1) (CAT#: ZG-0791J)
This product is a rabbit antibody that recognizes LGALS3.
Specific Inquiry
WB
Figure 1 Rabbit Anti-LGALS3 Antibody (ZG-0791J) in WB
Western Blot
Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, Colo320 whole cell lysate, HT29 whole cell lysate
All lanes: Galectin 3 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 27 kDa
Observed band size: 31 kDa
IHC
Figure 2 Rabbit Anti-LGALS3 Antibody (ZG-0791J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IF
Figure 3 Rabbit Anti-LGALS3 Antibody (ZG-0791J) in IF
Immunofluorescence staining of MCF7 Cells with this product at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
IP
Figure 4 Rabbit Anti-LGALS3 Antibody (ZG-0791J) in IP
Immunoprecipitating LGALS3 in Hela whole cell lysate
Lane 1: Rabbit control IgG in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0791J (2µg)+ Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (10µg)
Specifications
- Immunogen
- A synthesized peptide derived from human Galectin 3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human LGALS3
- Species Reactivity
- Human
- Clone
- 4E1
- Applications
- ELISA, WB, IHC, IF, IP
- Conjugate
- Uconjugated
- Related Disease
- Infections
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, IP:1:200-1:1000
Target
- Alternative Names
- L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2; galectin-3; 35 kDa lectin; IgE-binding protein; MAC-2 antigen; advanced glycation end-product receptor 3; carbohydrate-binding protein 35; epididymis secretory sperm binding protein; galactose-specific lectin 3; laminin-binding protein; lectin L-29; lectin, galactoside-binding, soluble, 3
- Gene ID
- 3958
- UniProt ID
- P17931
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "LGALS3"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1583z | Mouse Anti-LGALS3 Recombinant Antibody (clone 32D7) | WB, ELISA, FC, IHC, FuncS | Mouse IgG1 |
| MOB-244YJL | Rabbit Anti-LGALS3 Recombinant Antibody (clone CBL228) | WB, ELISA, FC, ICC, IF, IP | Rabbit IgG |
| MOB-245YJL | Mouse Anti-LGALS3 Recombinant Antibody (clone CBL229) | WB, ELISA, IP | Mouse IgG1 |
| ZG-0040C | Mouse Anti-LGALS3 Recombinant Antibody (clone 16E6) | IHC-P, IF, ELISA | Mouse IgG |
| ZG-0041C | Mouse Anti-LGALS3 Recombinant Antibody (clone 1H4) | IHC-P, IF, ELISA | Mouse IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2041 | Hi-Affi™ Recombinant Rabbit Anti-LGALS3 Monoclonal Antibody (DS2041AB) | FC, ICC, WB | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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