Rabbit Anti-LIG1 Recombinant Antibody (clone R02-5A2) (CAT#: VS3-FY2021)
The R02-5A2 recombinant monoclonal antibody is able to identify human LIG1. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. This LIG1 antibody has been teted positive by western blot analysis of LIG1 in PC-12 lysates.
Specific Inquiry
WB
Figure 1 Anti-LIG1 Antibody (VS3-FY2021) in WB.
The LIG1 expression in PC-12 lysates were detected using the anti-LIG1 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human LIG1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat LIG1
- Species Reactivity
- Human, Rat
- Clone
- R02-5A2
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 102 kDa, while the observed molecular weight stands at 102 kDa.
- Related Disease
- Immunodeficiency 96 and Bloom Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- LIGI; IMD96; hLig1
- Gene ID
- 3978
- UniProt ID
- P18858
- Sequence Similarities
- Belongs to the ATP-dependent DNA ligase family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitination at Lys226, Lys298, Lys407, and Lys795 (NX_P18858 [NX_P18858-1])
Modification sites at PhosphoSitePlus (P18858)
Glycosylation from GlyGen (P18858) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_047294789.1; XP_047294790.1; XP_054176994.1; XP_054176995.1; NP_000225.1
- Function
- DNA ligase that seals nicks in double-stranded during DNA repair.
Also involved in DNA replication and DNA recombination.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "LIG1"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3111z | Mouse Anti-LIG1 Recombinant Antibody (clone 11B4) | ELISA, IHC, WB | Mouse IgG2a, κ |
| MOB-0068CT | Recombinant Mouse anti-Human LIG1 Monoclonal antibody (20I6) | FC, ICC/IF, IP, WB | |
| MRO-0480-CN | Recombinant Rabbit Anti-LIG1 Monoclonal Antibody (JB43-39) | WB, IHC | Rabbit IgG |
| ZG-0052U | Rabbit Anti-LIG1 Recombinant Antibody (clone 7B2) | ELISA, WB, IHC | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2055 | Hi-Affi™ Recombinant Rabbit Anti-LIG1 Monoclonal Antibody (DS2055AB) | WB | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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