Recombinant monoclonal antibodies against the lipopolysaccharide of Ralstonia solanacearum (biovar 2, race 3) are provided. The antibody reacted with all race 3 strains tested, but with only some strains belonging to other races. Furthermore, only a few cross-reactions with saprophytic bacteria. Therefore, this antibody is potentially useful for detection of R. solanacearum race 3.
Figure 1 Reaction of purified monoclonal single-chain variable antibody fragments (scFv) (anti-LPS 3, anti-LPS 8, anti-LPS 12, and anti-LPS 13) with lipopolysaccharide (LPS) in enzyme-linked immunosorbent assay (ELISA).
ELISA plates were coated with LPS derived from Ralstonia solanacearum.
Griep, R. A., van Twisk, C., van Beckhoven, J. R., van der Wolf, J. M., & Schots, A. (1998). Development of Specific Recombinant Monoclonal Antibodies Against the Lipopolysaccharide of Ralstonia solanacearum Race 3. Phytopathology, 88(8), 795–803.
Figure 2 Immunofluorescence staining of whole Ralstonia solanacearum bacteria.
A surface epitope is recognized by single-chain variable antibody fragment (scFv) anti-lipopolysaccharide (LPS) 12.
Griep, R. A., van Twisk, C., van Beckhoven, J. R., van der Wolf, J. M., & Schots, A. (1998). Development of Specific Recombinant Monoclonal Antibodies Against the Lipopolysaccharide of Ralstonia solanacearum Race 3. Phytopathology, 88(8), 795–803.
Figure 3 Western blot showing that the epitope that is recognized is located on the LPS of R. solanacearum.
Purified LPS, derived from R. solanacearum (tracks 1 and 3) and R. picketti (tracks 2 and 4) was blotted onto nitrocellulose and stained with silver (Bio-Rad Laboratories) or with scFv anti-LPS 12 (tracks 3 and 4), respectively.
Griep, R. A., van Twisk, C., van Beckhoven, J. R., van der Wolf, J. M., & Schots, A. (1998). Development of Specific Recombinant Monoclonal Antibodies Against the Lipopolysaccharide of Ralstonia solanacearum Race 3. Phytopathology, 88(8), 795–803.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
NABL-060 | Recombinant Anti-V. cholerae O1 LPS VHH Single Domain Antibody | WB, ELISA, IP, FuncS | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
MHH-630 | Recombinant Human Anti-LPS Antibody | WB, IP, FuncS | IgG |
PABL-027 | Recombinant Mouse Anti-C. trachomatis LPS Antibody (SAG506-01) | WB, ELISA, FuncS | IgG |
FAMAB-0244-CN | Recombinant Chimeric (Mouse/Human) Anti-LPS Antibody (CK3) | WB, ELISA | Chimeric (mouse/human) IgG3, Κ |
FAMAB-0245-CN | Recombinant Mouse Anti-LPS Antibody (BML-5D11) | ELISA | Mouse IgG2b, λ |
FAMAB-0246-CN | Recombinant Mouse Anti-LPS Antibody (BML-18F8) | ELISA | Mouse IgG1, λ |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-027 | Recombinant Mouse Anti-C. trachomatis LPS Antibody Fab Fragment (SAG506-01) | WB, ELISA, FuncS | Fab |
PFBW-013 | Mouse Anti-LPS Recombinant Antibody (clone S45-18); Fab Fragment | WB, ELISA | Mouse Fab |
PFBW-168 | Mouse Anti-LPS Recombinant Antibody (clone F22-4); Fab Fragment | ELISA | Mouse Fab |
FAMAB-0244-CN-F(E) | Recombinant Chimeric (Mouse/Human) Anti-LPS Antibody Fab Fragment (CK3) | WB, ELISA | Chimeric (mouse/human) Fab |
FAMAB-0245-CN-F(E) | Recombinant Mouse Anti-LPS Antibody Fab Fragment (BML-5D11) | ELISA | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBW-013 | Mouse Anti-LPS Recombinant Antibody (clone S45-18); scFv Fragment | WB, ELISA | Mouse scFv |
PSBL-474 | Recombinant Mouse Anti-Ft LPS Antibody scFv Fragment (Ab-52) | WB, ELISA, FuncS | scFv |
FAMAB-0207-YC-S(P) | Recombinant Human Anti-LPS Antibody scFv Fragment (sc12) | ELISA, IF, WB | Human scFv |
FAMAB-0104-CN-S(P) | Recombinant Human Anti-LPS Antibody scFv Fragment (S20) | ELISA, FC, BL | Human scFv |
HPAB-J0169-YC-S(P) | Recombinant Human Anti-LPS Antibody scFv Fragment (216-01) | ELISA, FuncS | Human scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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