This product is a recombinant mouse antibody that recognizes LY75. The antibody was purified by affinity chromatography. It can be used in ELISA.
Figure 1 Analysis of DENV-specific antibodies in mice sera with ConA immobilized E glycoproteins.
Concanavalin A was used to immobilize DENV-2 from serum freevirus stocks and the reactivity evaluated with sera from different groups of immunized mice 45 days after boost. The results were analyzed by the Kruskal-Wallistest. Data are shown as mean ± SEM. **p-value <0.05 as compared to EDIII
Coconi-Linares, N., Ortega-Dávila, E., López-González, M., García-Machorro, J., García-Cordero, J., Steinman, R. M., ... & Gómez-Lim, M. A. (2013). Targeting of envelope domain III protein of DENV type 2 to DEC-205 receptor elicits neutralizing antibodies in mice. Vaccine, 31(19), 2366-2371.
Figure 2 Determination of neutralizing antibodies in sera from animals immunized with the different recombinant proteins employing PRNT50.
Neutralization titer 50was performed 20 days after the last immunization by infecting BHK-21 monolayers with DENV-2 and then incubating with mice sera. Individual serum sampleswere serially diluted from 1:5 to 1:80. Symbols represent individual mice and eachhorizontal line is the mean of experiments. **p-value <0.05 as compared to EDIII.
Coconi-Linares, N., Ortega-Dávila, E., López-González, M., García-Machorro, J., García-Cordero, J., Steinman, R. M., ... & Gómez-Lim, M. A. (2013). Targeting of envelope domain III protein of DENV type 2 to DEC-205 receptor elicits neutralizing antibodies in mice. Vaccine, 31(19), 2366-2371.
Figure 3 Proliferative T cell responses to DENV after immunization with recombinantproteins.
Mice were immunized with different recombinant proteins and 45 dayslater spleen cells were harvested and stimulated in vitro with scFvDEC205, E protein,DENV-2 and scFvDEC205-EDIII.
Coconi-Linares, N., Ortega-Dávila, E., López-González, M., García-Machorro, J., García-Cordero, J., Steinman, R. M., ... & Gómez-Lim, M. A. (2013). Targeting of envelope domain III protein of DENV type 2 to DEC-205 receptor elicits neutralizing antibodies in mice. Vaccine, 31(19), 2366-2371.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-637-S(P) | Recombinant Anti-human LY75 Antibody scFv Fragment | FC, Neut, Biosensors, FuncS | scFv |
MHH-637-S(P) | Recombinant Human Anti-human LY75 Antibody scFv Fragment | RIA, ELISA, FuncS | scFv |
HPAB-0432LY-S(P) | Human Anti-LY75 Recombinant Antibody; scFv Fragment (HPAB-0432LY-S(P)) | ELISA | Human scfv |
HPAB-0433LY-S(P) | Human Anti-LY75 Recombinant Antibody; scFv Fragment (HPAB-0433LY-S(P)) | ELISA | Human scfv |
HPAB-0661-YJ-S(P) | Mouse Anti-LY75 Recombinant Antibody (clone CBL70YJ); scFv Fragment | ELISA, IF | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MHH-637 | Recombinant Human Anti-human LY75 Antibody | WB, FC, FuncS | IgG |
MOB-079LC | Recombinant Mouse Anti-Bovine LY75 Antibody | FC | Mouse IgG |
MRO-0948-CN | Recombinant Rabbit Anti-LY75 Monoclonal Antibody (JB87-35) | WB, IHC, IP | Rabbit IgG |
FAMAB-1616CQ | Rat Anti-LY75 Recombinant Antibody (clone NLDC145) | IB, FC, IF, IHC | Rat IgG2a |
VS3-FY259 | Recombinant Mouse Anti-LY75 Antibody (clone 4B10E11) | ELISA, FC | Mouse IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-2105 | Hi-Affi™ Recombinant Rabbit Anti-LY75 Monoclonal Antibody (DS2105AB) | IHC-P, IHC-Fr | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0432LY-F(E) | Human Anti-LY75 Recombinant Antibody; Fab Fragment (HPAB-0432LY-F(E)) | ELISA | Human Fab |
HPAB-0433LY-F(E) | Human Anti-LY75 Recombinant Antibody; Fab Fragment (HPAB-0433LY-F(E)) | ELISA | Human Fab |
HPAB-0434LY-F(E) | Human Anti-LY75 Recombinant Antibody; Fab Fragment (HPAB-0434LY-F(E)) | ELISA | Human Fab |
HPAB-0435LY-F(E) | Human Anti-LY75 Recombinant Antibody; Fab Fragment (HPAB-0435LY-F(E)) | ELISA | Human Fab |
HPAB-0436LY-F(E) | Human Anti-LY75 Recombinant Antibody; Fab Fragment (HPAB-0436LY-F(E)) | ELISA | Human Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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