Rabbit Anti-MAPKAPK3 Recombinant Antibody (clone R08-4B6) (CAT#: VS3-FY2036)
The R08-4B6 recombinant monoclonal antibody has been genetically engineered to specifically target human MAPKAPK3. It is suitable for use in a variety of assays, including WB, IP. This MAPKAP kinase 3 antibody was used in western blot analysis of MK3 in A549, HL-60 lysates.
Specific Inquiry
WB
Figure 1 Anti-MAPKAPK3 Antibody (VS3-FY2036) in WB.
The MK3 expression in A549, HL-60 lysates were detected using the anti-MAPKAP Kinase 3 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human MK-3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MAPKAPK3
- Species Reactivity
- Human
- Clone
- R08-4B6
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 43 kDa, while the observed molecular weight stands at 43 kDa.
- Related Disease
- Macular Dystrophy, Patterned, 3 and Bacteremia 2
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Mitogen-Activated Protein Kinase-Activated Protein Kinase 3; MAPK-Activated Protein Kinase 3; Chromosome 3p Kinase; MAPKAP Kinase 3; EC 2.7.11.1; MAPKAP-K3; MAPKAPK-3
- Gene ID
- 7867
- UniProt ID
- Q16644
- Sequence Similarities
- Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylated and activated by MAPK1/ERK2 and MAPK3/ERK1.
Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-201, Ser-251 and Thr-313 ().
(Q16644-MAPK3_HUMAN)
Modification sites at PhosphoSitePlus (Q16644)
Modification sites at neXtProt (NX_Q16644)
Glycosylation from GlyGen (Q16644) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_047304839.1; XP_047304840.1; XP_047304841.1; XP_047304842.1; XP_047304843.1
- Function
- MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "MAPKAPK3"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1202CT | Recombinant Mouse anti-Human MAPKAPK3 Monoclonal antibody (EML2251) | WB |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0347MZ | Chicken Anti-MAPKAPK3 (ab1) Polyclonal IgY | WB | Chicken antibody |
| BRD-0348MZ | Chicken Anti-MAPKAPK3 (ab2) Polyclonal IgY | WB | Chicken antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2162 | Hi-Affi™ Recombinant Rabbit Anti-MAPKAPK3 Monoclonal Antibody (DS2162AB) | WB, FC, IP | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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