Rabbit Anti-MCM3 Recombinant Antibody (clone R04-6B9) (CAT#: VS3-FY1596)

• Immunogen
• Synthetic peptide of human MCM3

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human and Rat MCM3

• Species Reactivity
• Human, Rat

• Clone
• R04-6B9

• Applications
• Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence

• MW
• The calculated weight of the target protein is approximately 91 kDa, while the observed molecular weight stands at 100 kDa.

• Related Disease
• Anaplastic Astrocytoma and Cervical Cancer

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry: 1/50-1/100
  Immunofluorescence: 1/50-1/200

• Alternative Names
• Minichromosome Maintenance Complex Component 3; DNA Polymerase Alpha Holoenzyme-Associated Protein P1; RLF Subunit Beta; P1-MCM3; P102; MCM3 Minichromosome Maintenance Deficient 3 (S. Cerevisiae); Minichromosome Maintenance Deficient (S. Cerevisiae) 3; MCM3 Minichromosome Maintenance Deficient 3; Replication Licensing Factor, Beta Subunit; Minichromosome Maintenance Deficient 3

• Gene ID
• 4172

• UniProt ID
• P25205

• Sequence Similarities
• Belongs to the MCM family.

• Cellular Localization
• Chromosome, Nucleus

• Post Translation Modifications
• Acetylated by MCM3AP., O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

• Protein Refseq
• NP_001257401.1; NP_002379.3

• Function
• Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built.
  The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
  Required for the entry in S phase and for cell division (Probable).

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody