Rabbit Anti-MGMT Recombinant Antibody (clone 10G7) (CAT#: ZG-0467U)
Rabbit Anti-MGMT Antibody (clone 10G7) displayed high specificity against the MGMT protein of Human.
Specific Inquiry
WB
Figure 1 Rabbit Anti-MGMT Antibody (ZG-0467U) in WB
Western Blot
Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate
All lanes: MGMT antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
IHC
Figure 2 Rabbit Anti-MGMT Antibody (ZG-0467U) in IHC
IHC image of ZG-0467U diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human MGMT
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MGMT
- Species Reactivity
- Human
- Clone
- 10G7
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Oligodendroglioma and Idh-Wildtype Glioblastoma.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase; O6-methylguanine-DNA methyltransferase; methylguanine-DNA methyltransferase; EC 2.1.1.63
- Gene ID
- 4255
- UniProt ID
- P16455
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "MGMT"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2127z | Mouse Anti-MGMT Recombinant Antibody (clone 35A8) | WB, ELISA, IF, IHC | Mouse IgG1 |
| MOB-1163MZ | Recombinant Mouse Anti-Human MGMT Antibody (clone MT 23.2) | IHC-P | Mouse antibody |
| MRO-0995-CN | Recombinant Rabbit Anti-MGMT Monoclonal Antibody (CBACN-371) | WB, IHC | Rabbit IgG |
| MRO-2032-CN | Rabbit Anti-MGMT Polyclonal Antibody (MRO-2032-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-CJ702 | Mouse Anti-MGMT Recombinant Antibody (VS3-CJ702) | WB, IF, IHC-P | Mouse IgG1 |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0359MZ | Chicken Anti-MGMT Polyclonal IgY | WB | Chicken antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2228 | Hi-Affi™ Recombinant Rabbit Anti-MGMT Monoclonal Antibody (DS2228AB) | IHC-P, WB | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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