Popular Searches: Recombinant Antibody MHC Tetramer T cell Receptor Scaffold Protein
  • Home
  • MKI67
  • Mouse Anti-MKI67 Recombinant Antibody (clone ABT-KI67)

Mouse Anti-MKI67 Recombinant Antibody (clone ABT-KI67) (CAT#: VS3-CJ971)

This product is a mouse antibody that recognizes human MKI67.


See more Engineered Antibody products for MKI67
Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies & nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1164/13_B8_2_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/952_H5_3_selected.jpg
Figure 3 IF staining of human cell line A-431
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1164/30_B1_1_red_green.jpg
Normal Tissue
Figure 4 Colon
Figure 4 Colon
(Goblet cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_A_7_3.jpg
Figure 5 Testis
Figure 5 Testis
(Cells in seminiferous ducts
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_A_6_6.jpg
Figure 6 Skin
Figure 6 Skin
(Cells in basal layer
Staining: Medium
Intensity: Strong
Quantity: <25%
Cells in spinous layer
Staining: Medium
Intensity: Strong
Quantity: <25%
Hair follicles
Staining: High
Intensity: Strong
Quantity: 75%-25%
Lymphocytes
Staining: High
Intensity: Strong
Sebaceous glands
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_B_7_1.jpg
Figure 7 Lymph node
Figure 7 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000148773-MKI67
Figure 8 RNA cell line category: Low cell line specificity
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000148773-MKI67

Specifications

  • Immunogen
  • Synthesized peptide derived from human Ki-67
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1, κ
  • Specificity
  • Human MKI67
  • Species Reactivity
  • Human
  • Clone
  • ABT-KI67
  • Applications
  • IF, IHC-P
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Affinity Chromatography
  • Purity
  • >95% as determined by SDS-PAGE
  • Format
  • Liquid
  • Buffer
  • 50% Glycerol, 0.5% BSA, PBS.
  • Preservative
  • 0.02% Sodium Azide
  • Storage
  • Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Immunofluorescence (1:50-1:200), Immunohistochemistry-Parraffin (1:100-1:500).

Target

  • Alternative Names
  • KIA; MIB-; MIB-1; PPP1R105
  • Cellular Localization
  • Chromosome, Nucleus
  • Post Translation Modifications
  • Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604).
    Hyperphosphorylated form does not bind DNA.
  • Function
  • Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226).
    Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226).
    Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226).
    Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551).
    Does not contribute to the internal structure of mitotic chromosomes (By similarity).
    May play a role in chromatin organization (PubMed:24867636).
    It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).

Recommended Products

Secondary Antibody

Recommended Dilution Buffer

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "MKI67"

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2237 Hi-Affi™ Recombinant Rabbit Anti-MKI67 Monoclonal Antibody (DS2237AB) ELISA, WB, IHC IgG
MOR-4638 Hi-Affi™ Recombinant Rabbit Anti-MKI67 Monoclonal Antibody (TH151DS) IF, ICC, FC, ChIP IgG
MOR-0054-FY Rabbit Anti-MKI67 Recombinant Antibody (clone AFY0025) ICC, IHC-P, WB Rabbit IgG

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-2357LY-F(E) Mouse Anti-MKI67 Recombinant Antibody (clone 73043-24E8); Fab Fragment IHC, ELISA, WB Mouse Fab

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-2357LY-S(P) Mouse Anti-MKI67 Recombinant Antibody (clone 73043-24E8); scfv Fragment IHC, ELISA, WB Mouse scfv

Recombinant Antibody

CAT Product Name Application Type
ZG-0240C Mouse Anti-MKI67 Recombinant Antibody (ZG-0240C) IHC, ELISA Mouse IgG
ZG-0241C Mouse Anti-MKI67 Recombinant Antibody (ZG-0241C) WB, ELISA Mouse IgG
VS3-QX771 Mouse Anti-MKI67 Recombinant Antibody (clone 9C12B2) WB, IHC, ICC, FC, ELISA Mouse IgG1
VS3-XY1073 Mouse Anti-MKI67 Recombinant Antibody (clone 4A1) ELISA, WB, IHC Mouse IgG2b
VS3-XY1074 Mouse Anti-MKI67 Recombinant Antibody (clone 8D5) ELISA, WB, IHC Mouse IgG2b

Customer Reviews and Q&As

Submit a review or a question
There are currently no Customer reviews or questions for VS3-CJ971. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

© 2024 Creative Biolabs.
  • 0
  • 0
Cart
    Inquiry

    Go to compare