Rabbit Anti-MLLT10 Recombinant Antibody (clone R07-2G2) (CAT#: VS3-FY1641)
This product can be verification in WB, IHC assay. It is a rabbit recombinant monoclonal antibody that exhibits specific binding to human MLLT10. The recommended antibody dilution ratio is: western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5736/68_F4_2_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5736/68_F4_2_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5747/69_H4_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5747/69_H4_1_selected.jpg
Figure 3 IHC staining of human testis
(Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72790/171138_A_5_6_selected.jpg
(Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72790/171138_A_5_6_selected.jpg
Figure 4 High expression in testis
(Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5747/21104_A_6_6.jpg
(Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5747/21104_A_6_6.jpg
Figure 5 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000078403-MLLT10
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000078403-MLLT10
Specifications
- Immunogen
- Recombinant human AF10 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MLLT10
- Species Reactivity
- Human
- Clone
- R07-2G2
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 113 kDa, while the observed molecular weight stands at 140 kDa.
- Related Disease
- Leukemia, Acute Myeloid and Precursor T-Cell Acute Lymphoblastic Leukemia
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- MLLT10, Histone Lysine Methyltransferase DOT1L Cofactor; Myeloid/Lymphoid Or Mixed-Lineage Leukemia; Translocated To, 10; ALL1-Fused Gene From Chromosome 10 Protein; AF10; Myeloid/Lymphoid Or Mixed-Lineage Leukemia (Trithorax (Drosophila) Homolog); Translocated To, 10; Myeloid/Lymphoid Or Mixed-Lineage Leukemia (Trithorax Homolog, Drosophila); Translocated To, 10
- Gene ID
- 8028
- UniProt ID
- P55197
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Isopeptide bond, Phosphoprotein, Ubl conjugation
- Protein Refseq
- NP_001182555.1; NP_001182556.1; NP_001182557.1; NP_001182559.1; NP_001311225.1
- Function
- Probably involved in transcriptional regulation. in vitro or as fusion protein with KMT2A/MLL1 has transactivation activity. Binds to cruciform DNA. In cells, binding to unmodified histone H3 regulates DOT1L functions including histone H3 'Lys-79' dimethylation (H3K79me2) and gene activation.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "MLLT10"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0532MZ | Recombinant Mouse Anti-Human MLLT10 Antibody | WB | Mouse antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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