Mouse Anti-MMP14 (C-Terminus) Recombinant Antibody (VS3-CJ1005) (CAT#: VS3-CJ1005)

• Immunogen
• KLH conjugated synthetic peptide between 470-519 amino acids from the C-terminal region of human MMP14

• Host Species
• Mouse

• Type
• Mouse IgM

• Specificity
• Human MMP14 (C-Terminus)

• Species Reactivity
• Human

• Applications
• WB

• Conjugate
• Unconjugated

• Purification
• Protein A/G affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• PBS, pH 7.3.

• Preservative
• 0.09% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been tested for use in Western Blot (1:1000).

• Alternative Names
• MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1

• Gene ID
• 4323

• UniProt ID
• P50281

• Sequence Similarities
• Belongs to the peptidase M10A family.

• Cellular Localization
• Cytoplasm, Membrane

• Post Translation Modifications
• The precursor is cleaved by a furin endopeptidase.
  Tyrosine phosphorylated by PKDCC/VLK.

• Protein Refseq
• NP_004986.1

• Function
• Endopeptidase that degrades various components of the extracellular matrix such as collagen. Activates progelatinase A. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development (By similarity).
  May be involved in actin cytoskeleton reorganization by cleaving PTK7 (PubMed:20837484).
  Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro-MMP2 (PubMed:12714657).
  Cleaves ADGRB1 to release vasculostatin-40 which inhibits angiogenesis (PubMed:22330140).

Secondary Antibody

• 2ND-052CQ
• Goat Anti-Mouse IgM (H&L) Antibody

• 2RD-A1568-YJ
• Rabbit Anti-Mouse IgM Secondary antibody (Clone RM109)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS