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Rabbit Anti-NCS1 Recombinant Antibody (clone R35-9B-6) (CAT#: VS3-FY2139)

Clone R35-9B-6 is a rabbit recombinant monoclonal antibody that has been genetically engineered to specifically target human NCS1 protein, a calculated weight of 22 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R35-9B-6 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200), flow cytometry (dilution range 1:50).


See more Engineered Antibody products for NCS1
Specific Inquiry
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  • Fc Engineering:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19713/if_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18587/if_selected.jpg
Normal Tissue
Figure 3 High expression in cerebral cortex
Figure 3 High expression in cerebral cortex
(Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18587/40690_B_9_5.jpg
Figure 4 Colon
Figure 4 Colon
(Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18587/40690_A_9_3.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Figure 5 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107130-NCS1

Specifications

  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat NCS1
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R35-9B-6
  • Applications
  • Western Blot, Immunohistochemistry-Paraffin, Flow Cytometry
  • Conjugate
  • Unconjugated
  • MW
  • The calculated weight of the target protein is approximately 22 kDa, while the observed molecular weight stands at 22kDa.
  • Related Disease
  • Schizophrenia and Wolfram Syndrome 2

Product Property

  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS (pH 7.4), 150 mM NaCl, 50% glycerol
  • Preservative
  • 0.02% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry: 1/100-1/200
    Flow cytometry: 1/50

Target

  • Alternative Names
  • Neuronal Calcium Sensor 1; Frequenin-Like Ubiquitous Protein; Frequenin-Like Protein; Frequenin Homolog; FREQ; FLUP; Frequenin (Drosophila) Homolog; Frequenin Homolog (Drosophila); NCS-1
  • Sequence Similarities
  • Belongs to the recoverin family.
  • Cellular Localization
  • Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Synapse
  • Post Translation Modifications
  • Ubiquitination at Lys25 and Lys53 (NX_P62166 [NX_P62166-1])
    Modification sites at PhosphoSitePlus (P62166)
  • Function
  • Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin (By similarity).
    Stimulates PI4KB kinase activity (By similarity).

Recommended Products

Isotype Control

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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Recombinant Antibody

CAT Product Name Application Type
MOB-2290z Mouse Anti-NCS1 Recombinant Antibody (clone 22H11) ELISA, WB Mouse IgG2a, κ

Chicken IgY Antibody

CAT Product Name Application Type
BRD-1033MZ Chicken Anti-NCS1 Polyclonal IgY ELISA, IHC, WB Chicken antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2385 Hi-Affi™ Recombinant Rabbit Anti-NCS1 Monoclonal Antibody (DS2385AB) WB, IHC, IP, FC IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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