Rabbit Anti-NDUFAF1 Recombinant Antibody (clone R04-2J6) (CAT#: VS3-FY1787)
This R04-2J6 recombinant monoclonal antibody has been genetically engineered to specifically target human NDUFAF1. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. It is advised to dilute the sample for improved detectability by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). The binding specificity of the NDUFAF1 antibody was determined using western blot analysis of NDUFAF1 in K562, Hela lysates.
Specific Inquiry
WB
Figure 1 Anti-NDUFAF1 Antibody (VS3-FY1787) in WB.
The NDUFAF1 expression in K562, Hela lysates were detected using the anti-NDUFAF1 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human NDUFAF1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat NDUFAF1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-2J6
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 38 kDa, while the observed molecular weight stands at 35 kDa.
- Related Disease
- Mitochondrial Complex I Deficiency, Nuclear Type 11 and Isolated Complex I Deficiency
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- NADH:Ubiquinone Oxidoreductase Complex Assembly Factor 1; NADH Dehydrogenase (Ubiquinone) Complex I, Assembly Factor 1; CIA30; NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, Assembly Factor 1; NADH-Ubiquinone Oxidoreductase 1 Alpha Subcomplex, Assembly Factor 1; NADH Dehydrogenase [Ubiquinone] 1 Alpha Subcomplex Assembly Factor 1; Complex I Intermediate-Associated Protein 30, Mitochondrial; CGI-65; CGI65
- Gene ID
- 51103
- UniProt ID
- Q9Y375
- Sequence Similarities
- Belongs to the CIA30 family.
- Cellular Localization
- Mitochondrion
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q9Y375)
Modification sites at neXtProt (NX_Q9Y375)
- Protein Refseq
- XP_006720618.1; XP_011519960.1; XP_011519961.1; XP_024305713.1; XP_047288593.1
- Function
- As part of the MCIA complex, involved in the assembly of the mitochondrial complex I.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "NDUFAF1"
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2399 | Hi-Affi™ Recombinant Rabbit Anti-NDUFAF1 Monoclonal Antibody (DS2399AB) | FC, ICC, IHC-P, IP, WB | IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK373 | Rabbit Anti-NDUFAF1 Recombinant Antibody (clone JE47-18) | WB, ICC, IHC, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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