Rabbit Anti-NFIB Recombinant Antibody (clone R03-0E5) (CAT#: VS3-FY1800)

This R03-0E5 recombinant monoclonal antibody is specifically designed to target human NFIB. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IP. The binding specificity of the NFIB antibody was determined using western blot analysis of NFIB in C6, Hela lysates.


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WB

Figure 1 Anti-NFIB Antibody (VS3-FY1800) in WB.

Figure 1 Anti-NFIB Antibody (VS3-FY1800) in WB.

The NFIB expression in C6, Hela lysates were detected using the anti-NFIB by western blot assay.


Specifications

  • Immunogen
  • Synthetic peptides of human NFIB
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human and Rat NFIB
  • Species Reactivity
  • Human, Rat
  • Clone
  • R03-0E5
  • Applications
  • Western Blot, Immunoprecipitation
  • MW
  • The calculated weight of the target protein is approximately 47 kDa, while the observed molecular weight stands at 47,55 kDa.
  • Related Disease
  • Macrocephaly, Acquired, With Impaired Intellectual Development and Adenoid Cystic Carcinoma

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • Nuclear Factor I B; CCAAT-Box-Binding Transcription Factor; TGGCA-Binding Protein; Nuclear Factor I/B; Nuclear Factor 1/B; NF-I/B; NF1-B; NFI-B
  • Sequence Similarities
  • Belongs to the CTF/NF-I family.
  • Cellular Localization
  • Nucleus
  • Post Translation Modifications
  • Modification sites at PhosphoSitePlus (O00712)
    Modification sites at neXtProt (NX_O00712)
    Glycosylation from GlyGen (O00712) 4 sites, 1 O-linked glycan (4 sites)
  • Function
  • Transcriptional activator of GFAP, essential for proper brain development.
    Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication

Recommended Products

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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