Rabbit Anti-NKX2-1 Antibody (clone 6C11) displayed high specificity against the NKX2-1 protein of Human.
Figure 1 Rabbit Anti-NKX2-1 Antibody (ZG-0487U) in WB
Western Blot
Positive WB detected in: HepG2 whole cell lysate, U251 whole cell lysate, U87 whole cell lysate
All lanes: TTF1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
Figure 2 Rabbit Anti-NKX2-1 Antibody (ZG-0487U) in IHC
IHC image of ZG-0487U diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Figure 3 Rabbit Anti-NKX2-1 Antibody (ZG-0487U) in FC
Overlay histogram showing A549 cells stained with ZG-0487U (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10, 000 events was performed.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1310z | Mouse Anti-NKX2-1 Recombinant Antibody (clone 11B10) | IF, IHC, WB | Mouse IgG2b |
MOB-0342MZ | Recombinant Mouse Anti-Human NK2 Homeobox 1 Antibody (clone 9H8G4/2) | IHC | Mouse antibody |
ZG-0122C | Mouse Anti-NKX2-1 Recombinant Antibody (clone ABT-TTF1) | IHC-P, IF | Mouse IgG |
ZG-0123C | Mouse Anti-NKX2-1 Recombinant Antibody (clone PT2256) | IHC-P, IF | Mouse IgG |
VS3-FY1045 | Recombinant Rabbit Anti-NKX2-1 Antibody (clone R02-6A5) | WB, IHC-P, IP | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-2449 | Hi-Affi™ Recombinant Rabbit Anti-NKX2-1 Monoclonal Antibody (DS2449AB) | IHC-P | IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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