Rabbit Anti-NKX3-1 Recombinant Antibody (clone R08-9L5) (CAT#: VS3-XY3448)
This product is a recombinant rabbit antibody binding to NKX3-1 (NK3 homeobox 1), which acts as a tumor suppressor controlling prostate carcinogenesis. NKX3-1 plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. This antibody specifically reacts with human NKX3-1. It has been detected by Western Blot and Immunohistochemistry-Paraffin. The recommended dilutions of Western blot and Immunohistochemistry-Paraffin is 1/500-1/1000 and 1/50-1/100, respectively.
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 High expression in prostate
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78571/165671_A_1_5_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78571/165671_A_1_5_val_selected.jpg
Figure 2 Low expression in skin 1
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78571/165671_B_8_1_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78571/165671_B_8_1_val_selected.jpg
Figure 3 Testis
(Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78571/165671_A_6_6.jpg
(Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78571/165671_A_6_6.jpg
Figure 4 Prostate
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78571/165671_A_1_5.jpg
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78571/165671_A_1_5.jpg
Figure 5 RNA cell line category: Cell line enhanced (GAMG, SiHa, U-138 MG, U-251 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000167034-NKX3-1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000167034-NKX3-1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NKX3-1
- Species Reactivity
- Human
- Clone
- R08-9L5
- Applications
- WB, IHC-P
- Related Disease
- Rete Testis Neoplasm; Rete Testis Adenocarcinoma
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
Target
- Alternative Names
- NKX3; BAPX2; NKX3A; NKX3.1
- Gene ID
- 4824
- UniProt ID
- Q99801
- Long Name
- NK3 homeobox 1
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitinated by TOPORS; monoubiquitinated at several residues and also polyubiquitinated on single residues. (Q99801-NKX31_HUMAN)
Modification sites at PhosphoSitePlus (Q99801)
Modification sites at neXtProt (NX_Q99801)
- Protein Refseq
- NP_006158.2
- Function
- Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor.
Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate.
Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "NKX3-1"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1727MZ | Recombinant Mouse Anti-Human NKX3-1 Antibody | WB | Mouse antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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