Human Anti-NPM1 Recombinant Antibody (clone NA24) (CAT#: FAMAB-1229CQ)

Figure 1 Western blotting reactivity of monoclonal anti-NPM antibodies.

Wild-type NPM (38 kD) is detected in lysates of both normal cells (tonsil) and of the t(2;5)-positive cell line SU-DHL-1 with each of the three antibodies. However, only the two antibodies to the N-terminal region (NA24 and NPMa) react with the hybrid NPM-ALK protein (80 kD).

Cordell, J. L., Pulford, K. A., Bigerna, B., Roncador, G., Banham, A., Colombo, E., ... & Falini, B. (1999). Detection of normal and chimeric nucleophosmin in human cells. Blood, The Journal of the American Society of Hematology, 93(2), 632-642.

Figure 2 Western blotting reactivity of monoclonal anti-NPM antibodies.

The two antibodies to the N-terminal region(NA24 and NPMa) detect recombinant NPM-ALK and also recombinant NPM-MLF1 and NPM-RARa proteins. Antibody NPMc (againstthe C-terminal region) is unreactive with any of these fusion proteins.

Cordell, J. L., Pulford, K. A., Bigerna, B., Roncador, G., Banham, A., Colombo, E., ... & Falini, B. (1999). Detection of normal and chimeric nucleophosmin in human cells. Blood, The Journal of the American Society of Hematology, 93(2), 632-642.

Figure 3 Immunostaining of cytocentrifuged cell lines and paraffin-embedded normal tissues for NPM (APAAP technique).

Cell lines: The t(2;5)-positive cell line Karpas 299 (a) and a murine mastocytoma cell line (b) both show labeling of nucleoli with antibody to the C-terminal portion of NPM (antibody NPMc). Note the cytoplasmic labeling of a mitotic cell in (b). Kidney: (a) Many cells show nuclear labeling for nucleophosmin. (b) and (c) show high power views of, respectively, a glomerulus and tubular epithelium (antibody NA24). Thymus: Two different anti-NPM antibodies give essentially identical labeling patterns of cell nuclei in a Hassall's corpuscle and in the surrounding medullary tissue. Tonsil: Nuclear labeling of lymphoid cells is seen. The arrows in the low power view (left) indicate scattered mitotic cells in which there is cytoplasmic labeling, seen at high power on the right (antibody NA24). Cerebral cortex: Neural cells show strong cytoplasmic staining, seen at low power (above) and high power (below) (antibody NA24).

Cordell, J. L., Pulford, K. A., Bigerna, B., Roncador, G., Banham, A., Colombo, E., ... & Falini, B. (1999). Detection of normal and chimeric nucleophosmin in human cells. Blood, The Journal of the American Society of Hematology, 93(2), 632-642.

Figure 4 In a squamous cell carcinoma, the nuclei of all cells are labeled (antibody NA24 APAAP technique).

Occasional mitotic cells (arrows)show diffuse cytoplasmic labeling.

Cordell, J. L., Pulford, K. A., Bigerna, B., Roncador, G., Banham, A., Colombo, E., ... & Falini, B. (1999). Detection of normal and chimeric nucleophosmin in human cells. Blood, The Journal of the American Society of Hematology, 93(2), 632-642.