Rabbit Anti-NRAS Recombinant Antibody (VS3-CJ235) (CAT#: VS3-CJ235)
This product is a rabbit antibody that recognizes NRAS.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79912/1987_E10_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79912/1987_E10_1_selected.jpg
Figure 2 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10157/ihc_selected.jpg
(Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10157/ihc_selected.jpg
Figure 3 Cerebral cortex
(Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49830/118215_B_7_5.jpg
(Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49830/118215_B_7_5.jpg
Figure 4 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49830/118215_A_9_3.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49830/118215_A_9_3.jpg
Figure 5 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49830/118215_A_8_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49830/118215_A_8_8.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000213281-NRAS
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000213281-NRAS
Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat and Zebrafish NRAS
- Species Reactivity
- Human, Mouse, Rat, Zebrafish
- Applications
- WB, IP, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunoprecipitation, Flow Cytometry.
Target
- Alternative Names
- NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1; HRAS; KRAS
- Gene ID
- 4893
- UniProt ID
- P01111
- Sequence Similarities
- Belongs to the small GTPase superfamily. Ras family.
- Cellular Localization
- Cell membrane, Golgi apparatus, Membrane
- Post Translation Modifications
- Palmitoylated by the ZDHHC9-GOLGA7 complex (PubMed:16000296).
Depalmitoylated by ABHD17A, ABHD17B and ABHD17C (PubMed:26701913).
A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi (PubMed:16000296, PubMed:15705808, PubMed:2661017, PubMed:26701913).
Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).
Fatty-acylated at Lys-169 and/or Lys-170.
Ubiquitinated by the BCR(LZTR1) E3 ubiquitin ligase complex at Lys-170 in a non-degradative manner, leading to inhibit Ras signaling by decreasing Ras association with membranes.
Phosphorylation at Ser-89 by STK19 enhances NRAS-association with its downstream effectors.
(Microbial infection) Glucosylated at Thr-35 by P.sordellii toxin TcsL.
- Protein Refseq
- NP_002515.1
- Function
- Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "NRAS"
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2498 | Hi-Affi™ Recombinant Rabbit Anti-NRAS Monoclonal Antibody (DS2498AB) | WB, IP | IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1321-CN | Recombinant Rabbit Anti-NRAS Monoclonal Antibody (CBACN-478) | WB, IP, FC | Rabbit IgG |
| MRO-1322-CN | Recombinant Rabbit Anti-NRAS Monoclonal Antibody (JF10-11) | WB, IF, IP | Rabbit IgG |
| VS3-QX836 | Mouse Anti-NRAS Recombinant Antibody (clone 822CT17.5.1) | WB | Mouse IgG1 |
| VS3-CJ234 | Mouse Anti-NRAS Recombinant Antibody (VS3-CJ234) | WB | Mouse IgG1 |
| VS3-FY1069 | Recombinant Rabbit Anti-NRAS Antibody (clone R09-7E8) | WB, ICC, IF, IP | Rabbit IgG |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF595 | A*01:01/Human N-RAS (ILDTAGREEY) MHC Pentamer | FCM |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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