Rabbit Anti-NUDT5 Recombinant Antibody (clone R65-3J-2) (CAT#: VS3-FY2140)
This R65-3J-2 recombinant monoclonal antibody exhibits specific targeting for human NUDT5 protein, a calculated weight of 24 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R65-3J-2 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19827/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19827/if_selected.jpg
Figure 2 IHC staining of human liver
(Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19827/ihc_selected.jpg
(Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19827/ihc_selected.jpg
Figure 3 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19827/48121_A_7_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19827/48121_A_7_4.jpg
Figure 4 Kidney
(Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19827/48121_A_7_5.jpg
(Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19827/48121_A_7_5.jpg
Figure 5 Testis
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19827/48121_A_4_6.jpg
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19827/48121_A_4_6.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165609-NUDT5
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165609-NUDT5
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat NUDT5
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R65-3J-2
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 24 kDa, while the observed molecular weight stands at 30kDa.
- Related Disease
- Metabolism of nucleotides and Metabolism
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Target
- Alternative Names
- Nudix Hydrolase 5; Nudix (Nucleoside Diphosphate Linked Moiety X)-Type Motif 5; Nuclear ATP-Synthesis Protein NUDIX5; 8-Oxo-DGDP Phosphatase; HNUDT5; YSA1H; Nucleoside Diphosphate-Linked Moiety X Motif 5; ADP-Sugar Pyrophosphatase
- Gene ID
- 11164
- UniProt ID
- Q9UKK9
- Sequence Similarities
- Belongs to the Nudix hydrolase family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylation at Thr-45 is required for homodimer stability; dephosphorylation results in destabilization of the homodimer. Dephosphorylation at Thr-45 promotes the ATP-synthesis activity.
- Protein Refseq
- NP_054861.2
- Function
- Enzyme that can either act as an ADP-sugar pyrophosphatase in absence of diphosphate or catalyze the synthesis of ATP in presence of diphosphate.
In absence of diphosphate, hydrolyzes with similar activities various modified nucleoside diphosphates such as ADP-ribose, ADP-mannose, ADP-glucose, 8-oxo-GDP and 8-oxo-dGDP.
Can also hydrolyze other nucleotide sugars with low activity.
In presence of diphosphate, mediates the synthesis of ATP in the nucleus by catalyzing the conversion of ADP-ribose to ATP and ribose 5-phosphate. Nuclear ATP synthesis takes place when dephosphorylated at Thr-45.
Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming.
Does not play a role in U8 snoRNA decapping activity (By similarity).
Binds U8 snoRNA (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "NUDT5"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2209MZ | Recombinant Mouse Anti-Human NUDT5 Antibody | WB | Mouse antibody |
| VS4-CJ62 | Recombinant Rabbit Anti-NUDT5 Antibody (clone 10D2) | ELISA, WB, FC | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2516 | Hi-Affi™ Recombinant Rabbit Anti-NUDT5 Monoclonal Antibody (DS2516AB) | WB, IHC, FC | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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