The purified Rabbit Anti-NUP98 Recombinant Antibody (clone 2H4) displayed high specificity against the NUP98 protein of human.
Figure 1 Rabbit Anti-NUP98 Antibody (ZG-0018U) in WB
Positive WB detected in: K562 whole cell lysate, Hela whole cell lysate. All lanes: NUP98 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 198, 188, 98, 97, 196, 187 kDa
Observed band size: 100 kDa
Figure 2 Rabbit Anti-NUP98 Antibody (ZG-0018U) in IHC
IHC image of ZG-0018U diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Figure 3 Rabbit Anti-NUP98 Antibody (ZG-0018U) in IF
Immunofluorescence staining of Hela Cells with ZG-0018U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-2810z | Mouse Anti-NUP98 Recombinant Antibody (clone 32A9) | WB, IP | Mouse IgG1 |
ZG-0323C | Mouse Anti-NUP98 Recombinant Antibody (ZG-0323C) | WB | Mouse IgG |
ZG-0324C | Mouse Anti-NUP98 Recombinant Antibody (ZG-0324C) | WB | Mouse IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
PNBL-025 | Recombinant Anti-X. tropicalis Nup98 VHH Single Domain Antibody (TP377) | IF | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-2528 | Hi-Affi™ Recombinant Rabbit Anti-NUP98 Monoclonal Antibody (DS2528AB) | WB, IHC, ICC | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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