Rabbit Anti-OSBPL1A Recombinant Antibody (clone R02-2E2) (CAT#: VS3-FY1809)

This R02-2E2 recombinant monoclonal antibody is specifically directed against human OSBPL1A. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, ICC/IF, IP. This ORP1 antibody was used in western blot analysis of ORP1 in rat brain, C6, Hela lysates.


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WB

Figure 1 Anti-OSBPL1A Antibody (VS3-FY1809) in WB.

Figure 1 Anti-OSBPL1A Antibody (VS3-FY1809) in WB.

The ORP1 expression in rat Brain, C6, Hela lysates were detected using the anti-ORP1 by western blot assay.


Specifications

  • Immunogen
  • Synthetic peptide of human ORP1
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat OSBPL1A
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R02-2E2
  • Applications
  • Western Blot, Immunofluorescence, Immunoprecipitation
  • MW
  • The calculated weight of the target protein is approximately 108 kDa, while the observed molecular weight stands at 109,50 kDa.

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunofluorescence: 1/50-1/200
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • Oxysterol Binding Protein Like 1A; Oxysterol Binding Protein-Like 1B; OSBP-Related Protein 1; OSBPL1B; ORP-1; ORP1; Oxysterol-Binding Protein-Related Protein 1 Variant 1
  • Sequence Similarities
  • Belongs to the OSBP family.
  • Cellular Localization
  • Endosome
  • Post Translation Modifications
  • Modification sites at PhosphoSitePlus (Q9BXW6)
    Modification sites at neXtProt (NX_Q9BXW6)
    Glycosylation from GlyGen (Q9BXW6) 1 site, 1 O-linked glycan (1 site)
  • Function
  • Binds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate (By similarity).
    Stabilizes GTP-bound RAB7A on late endosomes/lysosomes and alters functional properties of late endocytic compartments via its interaction with RAB7A.
    Binds 25-hydroxycholesterol and cholesterol.

Recommended Products

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Rabbit Monoclonal Antibody

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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