Rabbit Anti-PDPN Recombinant Antibody (clone R77-1S-2) (CAT#: VS3-FY2118)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat PDPN

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R77-1S-2

• Applications
• Western Blot

• Conjugate
• Unconjugated

• MW
• The calculated weight of the target protein is approximately 17 kDa, while the observed molecular weight stands at 36kDa.

• Related Disease
• Lymphangioma and Angiosarcoma

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• PBS (pH 7.4), 150 mM NaCl, 50% glycerol

• Preservative
• 0.02% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000

• Alternative Names
• Podoplanin; Lung Type I Cell Membrane Associated Glycoprotein; Glycoprotein 36; PA2.26 Antigen; T1-Alpha; AGGRUS; GP36; T1A; Lung Type-I Cell Membrane-Associated Glycoprotein (T1A-2); Glycoprotein, 36-KD; HT1alpha-1

• Gene ID
• 10630

• UniProt ID
• Q86YL7

• Sequence Similarities
• Belongs to the podoplanin family.

• Cellular Localization
• Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane

• Post Translation Modifications
• Extensively O-glycosylated.
  Contains sialic acid residues.
  O-glycosylation is necessary for platelet aggregation activity.
  Disialylated at Thr-52; sialic acid is critical for platelet-aggregating activity and for CLEC1B interaction (PubMed:17222411, 25458834).
  (Q86YL7-PDPN_HUMAN)
  The N-terminus is blocked. (Q86YL7-PDPN_HUMAN)
  Cleaved by a metalloprotease within its extracellular (EC) domain, generating a membrane-bound C-terminal fragment (PCTF33) and an extracellular fragment.
  The resulting membrane-bound C-terminal fragment (PCTF33) is further processed between Val-150 and Val-151 by PSEN1/gamma-secretase generating the intracellular domain of podoplanin (PICD).

• Protein Refseq
• XP_006710358.1; XP_024307172.1; XP_047290427.1; XP_054189868.1; XP_054189869.1

• Function
• Through MSN or EZR interaction promotes epithelial-mesenchymal transition (EMT) leading to ERZ phosphorylation and triggering RHOA activation leading to cell migration increase and invasiveness.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody