Rabbit Anti-PIK3CB Recombinant Antibody (clone 5A9) (CAT#: ZG-0512U)

Rabbit Anti-PIK3CB Antibody (clone 5A9) displayed high specificity against the PIK3CB protein of Human.


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WB

Figure 1 Rabbit Anti-PIK3CB Antibody (ZG-0512U) in WB

Figure 1 Rabbit Anti-PIK3CB Antibody (ZG-0512U) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate
All lanes: PIK3CB antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 123 kDa
Observed band size: 110 kDa

IP

Figure 2 Rabbit Anti-PIK3CB Antibody (ZG-0512U) in IP

Figure 2 Rabbit Anti-PIK3CB Antibody (ZG-0512U) in IP

Immunoprecipitating PIK3CB in K562 whole cell lysate
Lane 1: Rabbit control IgG instead of ZG-0512U in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0512U(2µg)+ K562 whole cell lysate(500µg)
Lane 3: K562 whole cell lysate (10µg)


Specifications

  • Immunogen
  • A synthesized peptide derived from human PI3 Kinase p110 beta
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human PIK3CB
  • Species Reactivity
  • Human
  • Clone
  • 5A9
  • Applications
  • ELISA, WB, IP
  • Conjugate
  • Unconjugated
  • Related Disease
  • Nk-Cell Enteropathy and Immunodeficiency 14.

Product Property

  • Purification
  • Affinity chromatography purified
  • Buffer
  • PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunoprecipitation. Specific conditions should be optimized by the end user.
    WB:1:500-1:5000, IP:1:200-1:1000

Target

  • Alternative Names
  • Phosphatidylinositol 4;5-bisphosphate 3-kinase catalytic subunit beta isoform; PI3-kinase subunit beta; PI3K-beta; PI3Kbeta; PtdIns-3-kinase subunit beta; EC 2.7.1.153; Phosphatidylinositol 4;5-bisphosphate 3-kinase 110 kDa catalytic subunit beta; PtdIns-3-kinase subunit p110-beta; p110beta; PIK3CB; PIK3C1

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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