Rabbit Anti-PIK3CD Recombinant Antibody (clone R06-7A9) (CAT#: VS3-XY3465)
This is a primary rabbit antibody recognizing with PIK3CD (Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta), which plays a role in B-cell development, proliferation, migration, and function. The antibody specifically reacts with human, mouse and rat PIK3CD. It has been tested using Western Blot, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western blot and Immunofluorescence is 1/500-1/1000 and 1/50-1/200, respectively.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytokinetic bridge & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44953/847_C11_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytokinetic bridge & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44953/847_C11_2_selected.jpg
Figure 2 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15420/ihc_selected.jpg
(Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15420/ihc_selected.jpg
Figure 3 Testis
(Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44953/139742_A_6_6.jpg
(Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44953/139742_A_6_6.jpg
Figure 4 Appendix
(Lymphoid tissue Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44953/139742_A_3_2.jpg
(Lymphoid tissue Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44953/139742_A_3_2.jpg
Figure 5 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44953/139742_A_8_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44953/139742_A_8_8.jpg
Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, NB-4, REH, THP-1, U-937)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171608-PIK3CD
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171608-PIK3CD
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PIK3CD
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-7A9
- Applications
- WB, ICC, IF
- Related Disease
- Immunodeficiency 14A With Lymphoproliferation, Autosomal Dominant; Immunodeficiency 14B, Autosomal Recessive
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
Target
- Alternative Names
- APDS; PI3K; IMD14; p110D; IMD14A; IMD14B; ROCHIS; P110DELTA
- Gene ID
- 5293
- UniProt ID
- O00329
- Long Name
- Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity. (O00329-PK3CD_HUMAN)
Ubiquitination at Lys46 and Lys841 (NX_O00329 [NX_O00329-1])
Modification sites at PhosphoSitePlus (O00329)
Glycosylation from GlyGen (O00329) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_006710750.1
- Function
- Mediates immune responses.
Plays a role in B-cell development, proliferation, migration, and function.
Required for B-cell receptor (BCR) signaling.
Mediates B-cell proliferation response to anti-IgM, anti-CD40 and IL4 stimulation.
Promotes cytokine production in response to TLR4 and TLR9.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "PIK3CD"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1368z | Mouse Anti-PIK3CD Recombinant Antibody (clone 24D11) | WB, IP, IF, IHC, ELISA | Mouse IgG1, κ |
| MOB-2345CT | Recombinant Mouse anti-Human PIK3CD Monoclonal antibody (2H3) | WB, ICC | |
| MRO-1223-CN | Recombinant Rabbit Anti-PIK3CD Monoclonal Antibody (JM83-24) | WB, IHC | Rabbit IgG |
| VS3-WK615 | Rabbit Anti-PIK3CD Recombinant Antibody (clone JM83-24) | WB, ELISA | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2709 | Hi-Affi™ Recombinant Rabbit Anti-PIK3CD Monoclonal Antibody (DS2709AB) | WB | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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