Rabbit Anti-PPP1CA Recombinant Antibody (clone R04-8E4) (CAT#: VS3-FY1843)

• Immunogen
• Synthetic peptide of human PPP1CA+PPP1CB

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat PPP1CA

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R04-8E4

• Applications
• Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation

• MW
• The calculated weight of the target protein is approximately 38 kDa, while the observed molecular weight stands at 38 kDa.

• Related Disease
• Venezuelan Equine Encephalitis and Encephalitis

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry: 1/50-1/100
  Immunoprecipitation: 1/20

• Alternative Names
• PP1A, PP-1A, PPP1A, PP1alpha

• Gene ID
• 5499

• UniProt ID
• P62136

• Sequence Similarities
• Belongs to the PPP phosphatase family. PP-1 subfamily.

• Cellular Localization
• Cytoplasm, Nucleus

• Post Translation Modifications
• Phosphorylated. Dephosphorylated at Thr-320 in the presence of ionizing radiation.

• Protein Refseq
• NP_001008709.1; NP_002699.1; NP_996756.1

• Function
• Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca²⁺/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Regulates NEK2 function in terms of kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage. Regulator of neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective.
  Dephosphorylates CENPA.
  Dephosphorylates the 'Ser-139' residue of ATG16L1 causing dissociation of ATG12-ATG5-ATG16L1 complex, thereby inhibiting autophagy.
  (Microbial infection) Necessary for alphaviruses replication.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody