Rabbit Anti-PRC1 Recombinant Antibody (clone R04-3G7) (CAT#: VS3-FY1848)
The R04-3G7 recombinant monoclonal antibody is specific for human PRC1. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IHC-P, IP. This PRC1 antibody has been rigorously validated and is widely used in western blot analysis of PRC1 in C6, Jurkat lysates.
Specific Inquiry
WB
Figure 1 Anti-PRC1 Antibody (VS3-FY1848) in WB.
The PRC1 expression in C6, Jurkat lysates were detected using the anti-PRC1 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human PRC1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat PRC1
- Species Reactivity
- Human, Rat
- Clone
- R04-3G7
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 72 kDa, while the observed molecular weight stands at 72 kDa.
- Related Disease
- Bladder Cancer and Noonan Syndrome 3
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- ASE1; MAP65
- Gene ID
- 9055
- UniProt ID
- O43663
- Sequence Similarities
- Belongs to the MAP65/ASE1 family.
- Cellular Localization
- Chromosome, Cytoplasm, Cytoskeleton, Microtubule, Nucleus
- Post Translation Modifications
- Phosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules.
- Protein Refseq
- XP_005255044.1; XP_006720822.1; XP_006720823.1; XP_011520489.1; XP_011520490.1
- Function
- Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "PRC1"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0315CT | Recombinant Mouse anti-Saccharomyces cerevisiae PRC1 Monoclonal antibody (20B6C6) | ICC/IF, WB |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2834 | Hi-Affi™ Recombinant Rabbit Anti-PRC1 Monoclonal Antibody (DS2834AB) | IHC-P, WB, IHC-P, ICC/IF, FC, IP | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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