Rabbit Anti-PRKRA Recombinant Antibody (clone R04-8F7) (CAT#: VS3-FY1813)
This R04-8F7 recombinant monoclonal antibody has the ability to specifically bind to human PRKRA. It cross-reacts with mouse, rat. The specificity of the PRKRA antibody was confirmed by western blot analysis of PABPN1 in Jurkat, Hela, COS7, Raji lysates and immunohistochemistry analysis of paraffin-embedded human breast cancer.
Specific Inquiry
WB
Figure 1 Anti-PRKRA Antibody (VS3-FY1813) in WB.
The PABPN1 expression in Jurkat, Hela, COS7, Raji lysates were detected using the anti-PABPN1 by western blot assay.
IHC
Figure 2 Anti-PRKRA Antibody (VS3-FY1813) in IHC.
Immunohistochemistry staining of paraffin-embedded human breast cancer using the anti-PABPN1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Specifications
- Immunogen
- Synthetic peptide of human PACT (phospho-KR activating protein)/PRKRA
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PRKRA
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-8F7
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 34 kDa, while the observed molecular weight stands at 34 kDa.
- Related Disease
- Human breast cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- RAX; PACT; DYT16; HSD14
- Gene ID
- 8575
- UniProt ID
- O75569
- Sequence Similarities
- Belongs to the PRKRA family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Phosphorylated at Ser-246 in unstressed cells and at Ser-287 in stressed cells.
Phosphorylation at Ser-246 appears to be a prerequisite for subsequent phosphorylation at Ser-287.
Phosphorylation at Ser-246 and Ser-287 are necessary for activation of EIF2AK2/PKR under conditions of stress.
(O75569-PRKRA_HUMAN)
Modification sites at PhosphoSitePlus (O75569)
Modification sites at neXtProt (NX_O75569)
Glycosylation from GlyGen (O75569) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_011510365.1; XP_047302094.1; XP_054200276.1; XP_054200277.1; XP_054200278.1
- Function
- Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "PRKRA"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1528CT | Recombinant Mouse anti-Human PRKRA Monoclonal antibody (EML878) | IHC-P, WB |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2864 | Hi-Affi™ Recombinant Rabbit Anti-PRKRA Monoclonal Antibody (DS2864AB) | WB, IHC-P | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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