Rabbit Anti-PRMT3 Recombinant Antibody (clone R01-7K2) (CAT#: VS3-FY1852)

The R01-7K2 recombinant monoclonal antibody exhibits specific binding to human PRMT3. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-P, IP. Applications Tested: The PRMT3 antibody has been tested by western blot analysis of PRMT3 in rat brain, C6, 3T3, Hela lysates and immunohistochemistry analysis of paraffin-embedded human lung cancer.


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WB

Figure 1 Anti-PRMT3 Antibody (VS3-FY1852) in WB.

Figure 1 Anti-PRMT3 Antibody (VS3-FY1852) in WB.

The PRMT3 expression in rat Brain, C6, 3T3, Hela lysates were detected using the anti-PRMT3 by western blot assay.

IHC

Figure 2 Anti-PRMT3 Antibody (VS3-FY1852) in IHC.

Figure 2 Anti-PRMT3 Antibody (VS3-FY1852) in IHC.

Immunohistochemistry staining of paraffin-embedded human lung cancer using the anti-PRMT3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.


Specifications

  • Immunogen
  • Synthetic peptide of human PRMT3
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat PRMT3
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R01-7K2
  • Applications
  • Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
  • MW
  • The calculated weight of the target protein is approximately 60 kDa, while the observed molecular weight stands at 60 kDa.
  • Related Disease
  • Human lung cancer

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry: 1/50-1/100
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • HRMT1L3
  • Sequence Similarities
  • Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family.
  • Cellular Localization
  • Cytoplasm
  • Post Translation Modifications
  • Ubiquitination at Lys187 (NX_O60678 [NX_O60678-1])
    Modification sites at PhosphoSitePlus (O60678)
    Glycosylation from GlyGen (O60678) 1 site, 1 O-linked glycan (1 site)
  • Function
  • May regulate retinoic acid synthesis and signaling by inhibiting ALDH1A1 retinal dehydrogenase activity.

Recommended Products

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Recombinant Antibody

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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