Rabbit Anti-PRPF8 Recombinant Antibody (clone R06-1I7) (CAT#: VS3-FY1857)

• Immunogen
• Synthetic peptide of human PRPF8

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human PRPF8

• Species Reactivity
• Human

• Clone
• R06-1I7

• Applications
• Western Blot, Immunohistochemistry-Paraffin

• MW
• The calculated weight of the target protein is approximately 274 kDa, while the observed molecular weight stands at 274 kDa.

• Related Disease
• Retinitis Pigmentosa 13 and Retinitis Pigmentosa

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry: 1/50-1/100

• Alternative Names
• PRPF8; pre-mRNA processing factor 8; HPRP8; PRP8; PRPC8; RP13; SNRNP220

• Gene ID
• 10594

• UniProt ID
• Q6P2Q9

• Cellular Localization
• Nucleus, Spliceosome

• Post Translation Modifications
• Ubiquitination at Lys36, Lys43, Lys93, Lys218, Lys267, Lys278, Lys301, Lys428, Lys442, Lys452, Lys460, Lys480, Lys525, Lys609, Lys702, Lys705, Lys727, Lys746, Lys785, Lys932, Lys1020, Lys1158, Lys1210, Lys1222, Lys1300, Lys1344, Lys1392, Lys1434, Lys1443, Lys1449, Lys1463, Lys1732, Lys1735, Lys1792, Lys1801, Lys1859, Lys1866, Lys1901, Lys2034, Lys2049, Lys2070, and Lys2140 (NX_Q6P2Q9 [NX_Q6P2Q9-1])

• Protein Refseq
• XP_024306305.1; XP_054170697.1; XP_054185172.1; NP_006436.3

• Function
• Plays a role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes, both of the predominant U2-type spliceosome and the minor U12-type spliceosome. Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. Functions as a scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody